Daphnetin 瑞香素; 7,8-Dihydroxycoumarin|cas:486-35-1|西域试剂

Daphnetin 瑞香素; 7,8-Dihydroxycoumarin,99.86%

产品编号：Bellancom-N0281| CAS NO：486-35-1| 分子式：C₉H₆O₄| 分子量：178.14

Daphnetin (7,8-dihydroxycoumarin) 是从 Genus Daphne 中分离得到的一种香豆素衍生物，是一种蛋白酶 (protein kinase) 抑制剂，对 EGFR、PKA 和 PKC 的 IC50 值分别为 7.67 μM、9.33 μM 和 25.01 μM。Daphnetin (7,8-dihydroxycoumarin) 是用于抗炎、抗过敏疾病的植物次生代谢产物药物，也已被临床用于治疗凝血功能障碍、类风湿性关节炎等疾病，具有抗疟疾、解热等特性。

本网站销售的所有产品仅用于工业应用或者科学研究等非医疗目的,不可用于人类或动物的临床诊断或者治疗,非药用,非食用，

货号	价格	库存与货期
Bellancom-N0281	￥400.00	杭州北京（现货）
Bellancom-N0281	￥1600.00	杭州北京（现货）
Bellancom-N0281	￥2900.00	杭州北京（现货）

增值税发票√顺丰快递√订货电话：18601927057

Daphnetin 瑞香素; 7,8-Dihydroxycoumarin

产品介绍

Daphnetin (7,8-dihydroxycoumarin) 是一种香豆素衍生物，可来源于 Genus Daphne，是一种口服有效的蛋白激酶抑制剂 (protein kinase)，对 EGFR、PKA 和 PKC 的 IC₅₀ 值分别为 7.67 μM、9.33 μM 和 25.01 μM。Daphnetin 触发活性氧诱导的细胞凋亡 (apoptosis) 和通过调节 AMPK/Akt/mTOR 途径诱导细胞保护性自噬(autophagy)。Daphnetin 具有抗炎活性，并抑制 TNF-α、IL-1ß、ROS 和 MDA 的产生。Daphnetin 具有杀疟活性。瑞香素可用于类风湿关节炎、癌症和抗疟疾研究。

生物活性

Daphnetin (7,8-dihydroxycoumarin), one coumarin derivative can be found in plants of the Genus Daphne, is a potent, oral active protein kinase inhibitor, with IC₅₀s of 7.67 μM, 9.33 μM and 25.01 μM for EGFR, PKA and PKC in vitro, respectively. Daphnetin triggers ROS-induced cell apoptosis and induces cytoprotective autophagy by modulating the AMPK/Akt/mTOR pathway. Daphnetin has anti-inflammation activitity and inhibits TNF-α, IL-1ß, ROS, and MDA production. Daphnetin has schizontocidal activity against malaria parasites. Daphnetin can be used for rheumatoid arthritis , cancer and anti-malarian research^[4].

体外研究

Daphnetin (7,8-dihydroxycoumarin) (0-40 µg/mL; 24-48 hours) inhibits the proliferation of ovarian cancer cells.
Daphnetin (7,8-dihydroxycoumarin) (0-40 µg/mL; 24 hours; A2780 cells) induces apoptosis and increases ROS production in a dose-dependent manner.
Daphnetin (7,8-dihydroxycoumarin) (0-40 µg/mL; 24 hours; A2780 cells) induces autophagy through modulation of the AMPK/Akt/mTOR pathway.
Daphnetin (7,8-dihydroxycoumarin) (1-10 μM; plasmodium falciparum) exhibits schizontocidal activity in a dose-dependent manner.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay

Cell Line:	IOSE8C, A2780, SKOV3 and OVCAR8 cells
Concentration:	0, 5, 10, 20 and 40 µg/mL
Incubation Time:	24 h and 48 hours
Result:	Inhibited growth in ovarian cancer cells but not in normal cells.

Apoptosis Analysis

Cell Line:	A2780 and SKOV3 cells
Concentration:	0, 10, 20 and 40 µg/mL
Incubation Time:	24 hours
Result:	Increased apoptosis in a dose-dependent manner in A2780 and SKOV3 cells.

Western Blot Analysis

Cell Line:	A2780 and SKOV3 cells
Concentration:	0, 10, 20 and 40 µg/mL
Incubation Time:	24 hours
Result:	Increased proapoptotic protein (Caspase 3, Bax, and PARP) expression but decreased antiapoptotic protein (Bcl2) expression.

Western Blot Analysis

Cell Line:	A2780 cells
Concentration:	0, 10, 20 and 40 µg/mL
Incubation Time:	24 hours
Result:	Increased LC3 II and p62 expression in a dose-dependent manner and reduced the expression levels of p-Akt, p-mTOR, but increased the expression level of p-AMPK.

体内研究
(In Vivo)

Daphnetin (7,8-dihydroxycoumarin) (30 mg/kg; i.p.; daily; for 12 days; BALB/c nude mice) has antitumour activities in a xenograft animal model.
Daphnetin (7,8-dihydroxycoumarin) (2.5-10 mg/kg; i.p.; daily; for three days; C57BL/6 mice) inhibits cisplatin-induced inflammation, decreases TNF-α, IL-1β, ROS and MDA production in a dose-dependent manner in kidney tissues. Daphnetin inhibits cisplatin-induced NF-κB activation and up-regulated Nrf2 and HO-1.
Daphnetin (7,8-dihydroxycoumarin) (10-100 mg/kg; i.g. and i.p.; every four days, for 30 days; male Kunming outbred strain mice) displays certain schizontocidal activity in vivo.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	BALB/c nude mice
Dosage:	30 mg/kg
Administration:	Intraperitoneal injection; Daily; for 12 days
Result:	Decreased tumor volume and weight in a xenograft animal model.

Animal Model:	Male Kunming outbred strain mice
Dosage:	10, 50 or 100 mg/kg
Administration:	Oral gavage and intraperitoneal injection; every four days, for 30 days
Result:	Reduced the number of parasites in mice.

体内研究

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	BALB/c nude mice
Dosage:	30 mg/kg
Administration:	Intraperitoneal injection; Daily; for 12 days
Result:	Decreased tumor volume and weight in a xenograft animal model.

Animal Model:	Male Kunming outbred strain mice
Dosage:	10, 50 or 100 mg/kg
Administration:	Oral gavage and intraperitoneal injection; every four days, for 30 days
Result:	Reduced the number of parasites in mice.

体内研究

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	BALB/c nude mice
Dosage:	30 mg/kg
Administration:	Intraperitoneal injection; Daily; for 12 days
Result:	Decreased tumor volume and weight in a xenograft animal model.

Animal Model:	Male Kunming outbred strain mice
Dosage:	10, 50 or 100 mg/kg
Administration:	Oral gavage and intraperitoneal injection; every four days, for 30 days
Result:	Reduced the number of parasites in mice.

性状

Solid

溶解性数据

In Vitro:

DMSO : 50 mg/mL (280.68 mM; Need ultrasonic)

H₂O : 1 mg/mL (5.61 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	5.6136 mL	28.0678 mL	56.1356 mL
5 mM	1.1227 mL	5.6136 mL	11.2271 mL
10 mM	0.5614 mL	2.8068 mL	5.6136 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: ≥ 2.5 mg/mL (14.03 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (14.03 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。
将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液
2.

请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in saline)
Solubility: ≥ 2.5 mg/mL (14.03 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (14.03 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
3.

请依序添加每种溶剂： 10% DMSO 90% corn oil
Solubility: ≥ 2.5 mg/mL (14.03 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (14.03 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。

*以上所有助溶剂都可在西域网站选购。

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

参考文献

. Fan X, et, al. Daphnetin triggers ROS-induced cell death and induces cytoprotective autophagy by modulating the AMPK/Akt/mTOR pathway in ovarian cancer. Phytomedicine. 2021 Feb;82:153465. [Content Brief]

. Zhang L, et, al. Daphnetin protects against cisplatin-induced nephrotoxicity by inhibiting inflammatory and oxidative response. Int Immunopharmacol. 2018 Dec;65:402-407. [Content Brief]

. Wang QM, et, al. The schizontocidal activity of daphnetin against malaria parasites in vitro and in vivo. Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 2000;18(4):204-6. [Content Brief]

[4]. Yang EB, Zhao YN, Zhang K, Mack P. Daphnetin, one of coumarin derivatives, is a protein kinase inhibitor. Biochem Biophys Res Commun. 1999 Jul 14;260(3):682-5. [Content Brief]

化学品安全说明书(MSDS)

下载MSDS

质检证书(COA)

产品编号(Item Number)

批号(Lot Number)

个人防护装备	Eyeshields;Gloves;type N95 (US);type P1 (EN143) respirator filter
危害码 (欧洲)	Xi
风险声明 (欧洲)	R25
安全声明 (欧洲)	S22-S24/25
危险品运输编码	NONH for all modes of transport
WGK德国	2
RTECS号	GN6382600
海关编码	2942000000

1. 物质的识别

产品名：	Daphnetin
CAS号：	486-35-1
制造商/供应商：	西域试剂网站：www.hzbp.cn 邮件：13911702513@139.com

2. 合成/成分数据

产品名：	Daphnetin
别名：	7,8-Dihydroxycoumarin
分子式：	C9H6O4
分子量：	178.14

3. 急救措施

吸入后：	如果吸入，移至空气新鲜处，如果呼吸困难，给输氧，如呼吸停止，给予人工呼吸。
皮肤接触后：	用大量的水冲洗，移除污染的衣服和鞋子。
眼睛接触后：	检查并取下隐形眼镜，并用大量的水冲洗；呼叫医生。
吞食后：	如果吞食，用大量纯净水漱口；呼叫医生。

4. 消防措施

适当的灭火剂：	雾状水，二氧化碳，干粉或泡沫。
防护设备：	穿戴自给式呼吸器和防护服，以防止与皮肤和眼睛接触。

5. 泄漏应急处理

安全防范措施：	封锁泄漏区域；穿戴自给式呼吸器，防护服和厚橡胶手套。
清洁/收集措施：	使用液体粘合原料（硅藻土，通用粘合剂）吸取精细粉末；使用酒精擦洗表面和设备除去污渍；根据第11条处理被污染的材料。

6. 处理和储存

安全处理说明：	避免吸入和接触皮肤，眼睛及衣物；材料可能略微具有刺激性。
储存：	粉末型式 -20°C 3年；4°C 2年溶于溶剂 -80°C 6个月；-20°C 1个月

7. 接触控制和个人防护

呼吸设备：	NIOSH / MSHA认可的呼吸器。
双手保护：	耐化学腐蚀的橡胶手套。
眼睛防护：	化学安全护目镜。

8. 稳定性和反应活性

稳定性：	按照说明存储是稳定的；避免强氧化剂。
热分解/其他要避免的情况：	避免光和热。

9. 毒性资料

急性毒性：	无可用资料。
主要刺激性影响：	无可用资料。
在皮肤上：	无可用资料。
对眼睛：	无可用资料；可能具有刺激性。

10. 生态资料

一般注意事项：

无可用资料。

11. 废弃处置

按照所在国家，省份，县市和地方的法规处置。

12. 运输信息

正确的运输名称：	无
非危险品运输：	这种物质被视为非危险品运输。

13. 法规信息

尚未有针对此产品作出的化学安全性评估。

14. 其他信息

上游产品 10
617-48-1 87-66-1 104819-86-5 471-25-0
2445-80-9 19492-03-6 76474-93-6 108-24-7
110-16-7 2067-86-9
下游产品 6
19492-03-6 2445-80-9 21784-71-4 76474-93-6
483-92-1 485-90-5

18. [5.215] Tianrui Zhang et al."Daphnetin Improves Neuropathic Pain by Inhibiting the Expression of Chemokines and Inflammatory Factors in the Spinal Cord and Interfering with Glial Cell Polarization."Pharmaceuticals.2023 Feb;16(2):243

17. [IF=3.963] Liang Yang et al."Plant secondary metabolite, daphnetin reduces extracellular polysaccharides production and virulence factors of Ralstonia solanacearum."Pestic Biochem Phys. 2021 Aug;:104948

16. [IF=9.811] Manjie Gao et al."High-Crystallinity Covalent Organic Framework Synthesized in Deep Eutectic Solvent: Potentially Effective Adsorbents Alternative to Macroporous Resin for Flavonoids."Chem Mater. 2021;33(20):8036–8051

15. [IF=2.1] Xiaoying Chen et al."Effects of daphnetin combined with Bcl2‑siRNA on antiapoptotic genes in synovial fibroblasts of rats with collagen‑induced arthritis."Mol Med Rep. 2018 Jan;17(1):884-890

14. [IF=2.896] Junmei Li et al."Microwave-assisted extraction and ionic liquid-based dispersive liquid–liquid microextraction followed by HPLC for the determination of trace components in a Langdu preparation."Anal Methods-Uk. 2016 Jun;8(26):5218-5227

13. [IF=4.411] Liang Yang et al."New Insights into the Antibacterial Activity of Hydroxycoumarins against Ralstonia solanacearum."Molecules. 2016 Apr;21(4):468

12. [IF=4.464] Yamin Fu et al."A new calcium germanate-graphene nanocomposite modified electrode as efficient electrochemical sensor for determination of daphnetin."J Electroanal Chem. 2017 Sep;801:77

11. [IF=4.464] Shasha Jing et al."A sensitive sodium dodecyl sulfonate functionalized graphene hybrid SnO2 nanoparticles composite modified glassy carbon electrode for detecting daphnetin."J Electroanal Chem. 2017 Feb;787:72

10. [IF=4.464] Wenhua Qiao et al."Electrochemical behavior of daphnetin and its sensitive determination based on electrochemically reduced graphene oxide modified electrode."J Electroanal Chem. 2015 Jul;749:68

9. [IF=5.64] Wu Dousheng et al."Oleanolic Acid Induces the Type III Secretion System of Ralstonia solanacearum."Front Microbiol. 2015 Dec;0:1466

8. Yamin Fu, Lu Wang, Di Huang, Lina Zou, Baoxian Ye, A new calcium germanate-graphene nanocomposite modified electrode as efficient electrochemical sensor for determination of daphnetin, Journal of Electroanalytical Chemistry, Volume 801, 2017, Pages 77-83,

7. Shasha Jing, Huijun Zheng, Li Zhao, Lingbo Qu, Lanlan Yu, A sensitive sodium dodecyl sulfonate functionalized graphene hybrid SnO2 nanoparticles composite modified glassy carbon electrode for detecting daphnetin, Journal of Electroanalytical Chemistry, Vol

6. Mohammadi, Abdorreza, et al. "Enzyme-assisted extraction and ionic liquid-based dispersive liquid–liquid microextraction followed by high-performance liquid chromatography for determination of patulin in apple juice and method optimization using central co

5. 王玲,杨心悦,刘珂,陈明晰,徐新花.PSS功能化海绵状石墨烯电化学传感灵敏检测瑞香素的研究[J].分析测试学报,2020,39(08):1012-1017.

4. 姚洪娟, 刘闯, 兰晓琪,等. 长白瑞香的生药学研究[J]. 中药材, 2019(2).

3. 代婵, 王洁莹, 邓慧敏,等. 瑞香素在TNF-α诱导的CIA大鼠成纤维样滑膜细胞NF-κB信号通路中的抑炎作用[J]. 时珍国医国药, 2018, v.29;No.269(01):20-24.

2. 刘闯, 姚洪娟, 姜路晴,等. 基于HPLC指纹图谱结合化学模式识别的长白瑞香质量控制研究[J]. 中药材, 2019(10).

1. 王玲杨心悦刘珂等. PSS功能化海绵状石墨烯电化学传感灵敏检测瑞香素的研究[J]. 分析测试学报 2020(8).

Daphnetin 瑞香素; 7,8-Dihydroxycoumarin,99.86%

Daphnetin 瑞香素; 7,8-Dihydroxycoumarin

化学品安全说明书(MSDS)

质检证书(COA)

1. 物质的识别

2. 合成/成分数据

3. 急救措施

4. 消防措施

5. 泄漏应急处理

6. 处理和储存

7. 接触控制和个人防护

8. 稳定性和反应活性

9. 毒性资料

10. 生态资料

11. 废弃处置

12. 运输信息

13. 法规信息

14. 其他信息

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