Liperfluo, a marker of ferroptosis, is a useful fluorescent probe for investigating the roles of lipid peroxidation in a variety of cell pathophysiologies. Liperfluo reduces lipid hydroperoxides to lipid alcohols and is used for imaging lipid hydroperoxides in living cells.

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. 玻璃底培养皿中的细胞在 RPMI 1640 中用 50 µM H₂O₂ 在 37 °C 下处理 2 小时。去除 H₂O₂ 后，细胞用汉克氏平衡盐溶液 (HBSS) 洗涤 3 次。
2. 在 37°C 下用 10 µM Liperfluo 在 HBSS 中处理细胞 30 分钟。去除 Liperfluo 后，细胞用 HBSS 洗涤 3 次。
3. 使用 BZ-8000 荧光显微镜从 3 个独立的培养皿中获取荧光图像。

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

• . Kazuo Tomita, et al. MiR-7-5p Is Involved in Ferroptosis Signaling and Radioresistance Thru the Generation of ROS in Radioresistant HeLa and SAS Cell Lines. Int J Mol Sci. 2021 Aug 2;22(15):8300.  [Content Brief]

  . Elizabeth M Kenny, et al. Ferroptosis Contributes to Neuronal Death and Functional Outcome After Traumatic Brain Injury. Crit Care Med. 2019 Mar;47(3):410-418.  [Content Brief]

  . Kazunori Yamanaka, et al. A novel fluorescent probe with high sensitivity and selective detection of lipid hydroperoxides in cells. RSC Advances, 2012, 2, 7894–7900.