Naph-EA-mal Thiol-green 1|cas:210292-65-2|西域试剂

Naph-EA-mal Thiol-green 1,95.0%

产品编号：Bellancom-D1261| CAS NO：210292-65-2| 分子式：C₂₂H₂₁N₃O₄| 分子量：391.42

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货号	包装	价格	库存与货期	购买量	操作
Bellancom-D1261		￥3000.00	杭州北京（现货）
Bellancom-D1261		￥4800.00	杭州北京（现货）

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Naph-EA-mal Thiol-green 1

产品介绍

Naph-EA-mal (Thiol-green 1) 是一种快速检测和超快 turn-on 型硫醇 (thiol) 荧光探针，可用于蛋白标记和生物成像。Naph-EA-mal (Thiol-green 1) 可用于检测活细胞中的硫醇，标记蛋白质硫醇，定量细胞裂解液中总硫醇的浓度，并确定固定细胞中可逆的蛋白质硫醇氧化。Ex: 488 nM; Em: 540 nM。

生物活性

Naph-EA-mal (Thiol-green 1) is a rapid detect and ultrafast turn-on thiol fluorescence probe for protein labeling and bioimaging. Naph-EA-mal (Thiol-green 1) can be used to detect thiols in living cells, label the protein thiols, quantify the concentration of total thiols in cell lysate, and determine the reversible protein thiols oxidation in fixed cells. Ex: 488 nM; Em: 540 nM.

体外研究

Imaging thiols in living cells
Hep G2 cells incubated with Naph-EA-mal (Thiol-green 1) (1 µM;5 mins), and strong green fluorescence can be observed. When cells are preincubated the cells to block the intracellular thiols with NEM (a thiol block reagent; 100 µM), a weaker fluorescence is observed in cells.
Monitoring thiol-disulfide levels in fixed cells
Due to the majority of cellular thiols are present in the reduced form, Naph-EA-mal (Thiol-green 1) (10 µM) induces strong fluorescence in Hep G2 cells. After diamide treatment, fluorescent signal sharp dimed is observed.
The Hep G2 cells, oxidized by diamide (200 µM; 30 min), are fixed by 70% EtOH and the remaining free thiols are blocked by NEM (100 µM). then The cells are further treated with TCEP (5 mM) followed by addition of the Naph-EA-mal (Thiol-green 1) (10 µM). The green fluorescent signal is detected again

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究

性状

Solid

溶解性数据

In Vitro:

DMSO : 33.33 mg/mL (85.15 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	2.5548 mL	12.7740 mL	25.5480 mL
5 mM	0.5110 mL	2.5548 mL	5.1096 mL
10 mM	0.2555 mL	1.2774 mL	2.5548 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式