4-Di-10-ASP|cas:95378-73-7|西域试剂

4-Di-10-ASP,98.0%

产品编号：Bellancom-D1630| CAS NO：95378-73-7| 分子式：C₃₄H₅₅IN₂| 分子量：618.72

4-Di-10-ASP是一种荧光亲脂示踪剂（激发485 nm；发射620 nm）。4-Di-10-ASP可用于以特定方式染色磷脂膜。

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货号	价格	库存与货期
Bellancom-D1630	￥2800.00	杭州北京（现货）
Bellancom-D1630	￥4500.00	杭州北京（现货）
Bellancom-D1630	￥9400.00	杭州北京（现货）
Bellancom-D1630	￥15000.00	杭州北京（现货）

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4-Di-10-ASP

产品介绍

4-Di-10-ASP 是一种荧光亲脂性示踪剂 (Excitation 485 nm; Emission 620 nm)。4-Di-10-ASP 可以用来对磷脂膜进行特殊染色。

生物活性

4-Di-10-ASP is a fluorescent lipophilic tracer (Excitation 485 nm; Emission 620 nm). 4-Di-10-ASP can be used to stain phospholipid membranes in a specific manner.

体外研究

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. 4-Di-10-ASP (1 μM), DOPC (HY-113424A) (10 mM), and DOPG (HY-142980) (1 mM) is dissolved in methanol/chloroform (25 L, 1:2 v/v).
2. The solution is allowed to dry overnight under vacuum to obtain lamellar lipid films, which in turn are hydrated with the transcription/translation solution (25 L) for three hours at 37℃.
3. An aliquot (10 L) of the solution thus prepared is placed on a glass slide and sealed by a cover glass.
4. The sample is immediately observed with a confocal laser-scanning microscope, an argon laser (488 nm) is employed to excite the 4-Di-10-ASP.

体内研究

性状

Solid

溶解性数据

In Vitro:

DMSO : 33.33 mg/mL (53.87 mM; ultrasonic and warming and heat to 60°C)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	1.6162 mL	8.0812 mL	16.1624 mL
5 mM	0.3232 mL	1.6162 mL	3.2325 mL
10 mM	0.1616 mL	0.8081 mL	1.6162 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

参考文献

. Z J Huang, et al. Partition coefficients of fluorescent probes with phospholipid membranes. Biochem Biophys Res Commun. 1991 Nov 27;181(1):166-71. [Content Brief]

. Shin-ichiro M Nomura, et al. Gene expression within cell-sized lipid vesicles. Chembiochem. 2003 Nov 7;4(11):1172-5. [Content Brief]

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4-Di-10-ASP,98.0%

4-Di-10-ASP

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