NSC668394|cas:382605-72-3|西域试剂

NSC668394,99.80%

产品编号：Bellancom-115492| CAS NO：382605-72-3| 分子式：C₁₇H₁₂Br₂N₂O₃| 分子量：452.10

本网站销售的所有产品仅用于工业应用或者科学研究等非医疗目的,不可用于人类或动物的临床诊断或者治疗,非药用,非食用，

货号	价格	库存与货期
Bellancom-115492	￥1200.00	杭州北京（现货）
Bellancom-115492	￥1800.00	杭州北京（现货）
Bellancom-115492	￥3700.00	杭州北京（现货）
Bellancom-115492	￥6000.00	杭州北京（现货）
Bellancom-115492	￥9800.00	杭州北京（现货）

增值税发票√顺丰快递√订货电话：18601927057

NSC668394

产品介绍

NSC668394 是一种有效的 ezrin (Thr567) 磷酸化抑制剂，K_d 值为 12.59 μM。NSC668394 主要通过与 ezrin 结合来抑制 PKCΙ 导致的 ezrin T567 磷酸化。NSC668394 可用于预防肿瘤转移。

生物活性

NSC668394 is a potent ezrin (Thr567) phosphorylation inhibitor, with a K_d of 12.59 μM. NSC668394 inhibit ezrin T567 phosphorylation caused by PKCΙ primarily via their binding to ezrin. NSC668394 can be used to prevent tumor metastasis.

体外研究

NSC668394 (10 μM; pretreated for 15 min) inhibits ezrin T567 phosphorylation (IC₅₀=8.1 μM) and actin binding in vitro.
NSC668394 (1-10 μM; 2-6 h) inhibits ezrin-mediated invasion by K7M2 osteosarcoma (OS) cells on the HUVEC monolayer.
NSC668394 (20 μM) causes significant decrease in growth in JM1 and JM2 rat hepatoma cell lines.
NSC668394 (10 μM) reduces cell motility phenotypes in zebrafish.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis

Cell Line:	K7M2 OS cells
Concentration:	10 μM
Incubation Time:	6 hours
Result:	Inhibited T567 phosphorylation and actin binding of endogenous ezrin without altering cellular ezrin levels.

体内研究
(In Vivo)

NSC668394 (0.226 mg/kg/day; i.p. 5-days a week) inhibits ezrin-dependent in vivo OS metastatic growth in mouse lung.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Female BALB/c and SCID/Beige mice were injected with K7M2 or MNNG-HOS tumor cells
Dosage:	0.226 mg/kg/day
Administration:	I.p. 5-days a week for 66 days
Result:	Showed an increase in survival. Decreased the number of the green fluorescent protein (GFP)-expressing metastatic foci in the lung tissues.

体内研究

NSC668394 (0.226 mg/kg/day; i.p. 5-days a week) inhibits ezrin-dependent in vivo OS metastatic growth in mouse lung.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Female BALB/c and SCID/Beige mice were injected with K7M2 or MNNG-HOS tumor cells
Dosage:	0.226 mg/kg/day
Administration:	I.p. 5-days a week for 66 days
Result:	Showed an increase in survival. Decreased the number of the green fluorescent protein (GFP)-expressing metastatic foci in the lung tissues.

体内研究

NSC668394 (0.226 mg/kg/day; i.p. 5-days a week) inhibits ezrin-dependent in vivo OS metastatic growth in mouse lung.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Female BALB/c and SCID/Beige mice were injected with K7M2 or MNNG-HOS tumor cells
Dosage:	0.226 mg/kg/day
Administration:	I.p. 5-days a week for 66 days
Result:	Showed an increase in survival. Decreased the number of the green fluorescent protein (GFP)-expressing metastatic foci in the lung tissues.

性状

Solid

溶解性数据

In Vitro:

DMSO : 50 mg/mL (110.60 mM; ultrasonic and warming and heat to 60°C)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	2.2119 mL	11.0595 mL	22.1190 mL
5 mM	0.4424 mL	2.2119 mL	4.4238 mL
10 mM	0.2212 mL	1.1059 mL	2.2119 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: ≥ 2.5 mg/mL (5.53 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (5.53 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。
将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液