Skp2 Inhibitor C1 SKPin C1|cas:432001-69-9|西域试剂

Skp2 Inhibitor C1 SKPin C1,98.21%

产品编号：Bellancom-16661| CAS NO：432001-69-9| 分子式：C₁₈H₁₃BrN₂O₄S₂| 分子量：465.34

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货号	包装	价格	库存与货期	购买量	操作
Bellancom-16661		￥1400.00	杭州北京（现货）
Bellancom-16661		￥5880.00	杭州北京（现货）

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Skp2 Inhibitor C1 SKPin C1

产品介绍

Skp2 Inhibitor C1 (SKPin C1) 是一种 S 相激酶相关蛋白 2 (Skp2) 抑制剂，对转移性黑色素瘤细胞具有抑制作用。Skp2 Inhibitor C1 能够减缓细胞周期，抑制细胞增殖，并诱导凋亡 (apoptosis)。

生物活性

Skp2 Inhibitor C1 (SKPin C1) is an S-phase kinase-related protein 2 (Skp2) inhibitor with an inhibitory effect on metastatic melanoma cells. Skp2 Inhibitor C1 slows the cell cycle, inhibits cell proliferation, and triggers apoptosis.

体外研究

Skp2 Inhibitor C1 (10-50 μM; 12 hr) decreases the viability of THP-1, U266 and RPMI 8226 cells.
Skp2 Inhibitor C1 (25 μM) increases p27 protein levels in U266 and RPMI 8226 cells by inhibiting ubiquitination.
Skp2 Inhibitor C1 (25 μM) inhibits cell cycle of U266 and RPMI 8226 cells.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cycle Analysis

Cell Line:	B lymphocytes, THP-1, U266 and RPMI 8226 cells
Concentration:	0, 5, 10, 25, and 50 μM
Incubation Time:	12 hr, 24 hr, 36 hr, and 48 hr
Result:	Significantly decreased the viability of U266 and RPMI 8226 cells at 10 μM for 12 hours. Did not significantly affect B lymphocyte viability at 50 μM. Decreased THP-1 cell viability at 50 μM for 12 hours.

Cell Viability Assay

Cell Line:	U266 and RPMI 8226 cells
Concentration:	0, 5, 10, 25, and 50 μM
Incubation Time:	12 hr
Result:	Increased the percentages of U266 and RPMI 8226 cells in the G0/G1 phase, while decreased the percentages in S and G2/M phases.

体内研究
(In Vivo)

Skp2 Inhibitor C1 (5 mg/kg and 10 mg/kg; 3 times within 24 h: 24, 5, and 1 h before the test) shows the antidepressant-like effect in mouse models following chronic treatment by using the tail suspension test (TST), forced swimming test (FST), and social interaction test (SIT).

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

性状

Solid

溶解性数据

In Vitro:

DMSO : 20.83 mg/mL (44.76 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	2.1490 mL	10.7448 mL	21.4897 mL
5 mM	0.4298 mL	2.1490 mL	4.2979 mL
10 mM	0.2149 mL	1.0745 mL	2.1490 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in saline)
Solubility: ≥ 2.08 mg/mL (4.47 mM); Clear solution

此方案可获得 ≥ 2.08 mg/mL (4.47 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明