KCC-07|cas:315702-75-1|西域试剂

KCC-07,99.57%

产品编号：Bellancom-131031| CAS NO：315702-75-1| 分子式：C₁₄H₁₁N₃OS| 分子量：269.32

本网站销售的所有产品仅用于工业应用或者科学研究等非医疗目的,不可用于人类或动物的临床诊断或者治疗,非药用,非食用，

货号	价格	库存与货期
Bellancom-131031	￥850.00	杭州北京（现货）
Bellancom-131031	￥1500.00	杭州北京（现货）
Bellancom-131031	￥3000.00	杭州北京（现货）
Bellancom-131031	￥4800.00	杭州北京（现货）
Bellancom-131031	￥7500.00	杭州北京（现货）

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KCC-07

产品介绍

KCC-07 是一种有效的，选择性的且可透过血脑屏障的 MBD2 (methyl-CpG-binding domain protein 2) 抑制剂。KCC-07 防止 MBD2 与甲基化 DNA 结合，并激活脑特异性血管生成抑制剂 1 (BAI1) 诱导抗增殖 BAI1/p53/p21 信号传导。抗癌活性。

生物活性

KCC-07 is a potent, selective and brain-penetrant MBD2 (methyl-CpG-binding domain protein 2) inhibitor. KCC-07 prevents binding of MBD2 to methylated DNA and activates brain specific angiogenesis inhibitor 1 (BAI1) inducing anti-proliferative BAI1/p53/p21 signaling. Anticancer activity.

体外研究

KCC-07 (10 μM; 72 hours; MB cells) treatment clearly inhibited MB cell growth in vitro, consistent with induction of anti-proliferative BAI1/p53/p21 signaling.
KCC-07 (10 μM; 48 hours; MB cells) treatment largely abrogates MBD2 binding to the ADGRB1 promoter and restores BAI1 mRNA and protein expression in BAI1-silent MB cells.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay

Cell Line:	Medulloblastomas (MB) cells
Concentration:	10 μM
Incubation Time:	72 hours
Result:	Clearly inhibited MB cell growth in vitro.

Western Blot Analysis

Cell Line:	Medulloblastomas (MB) cells
Concentration:	10 μM
Incubation Time:	48 hours
Result:	Largely abrogated MBD2 binding to the ADGRB1 promoter in BAI1-silent MB cells.

体内研究
(In Vivo)

KCC-07 (100 mg/kg; intraperitoneal injection; 5 days/week; athymic nude mice) treatment inhibits tumor growth and significantly extends the survival of MB xenografts in vivo.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Outbred athymic nude mice (females; 8-10 weeks old) injected with MB cells
Dosage:	100 mg/kg
Administration:	Intraperitoneal injection; 5 days/week
Result:	Significantly extended the survival of MB xenografts in vivo.

体内研究

KCC-07 (100 mg/kg; intraperitoneal injection; 5 days/week; athymic nude mice) treatment inhibits tumor growth and significantly extends the survival of MB xenografts in vivo.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Outbred athymic nude mice (females; 8-10 weeks old) injected with MB cells
Dosage:	100 mg/kg
Administration:	Intraperitoneal injection; 5 days/week
Result:	Significantly extended the survival of MB xenografts in vivo.

体内研究

KCC-07 (100 mg/kg; intraperitoneal injection; 5 days/week; athymic nude mice) treatment inhibits tumor growth and significantly extends the survival of MB xenografts in vivo.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Outbred athymic nude mice (females; 8-10 weeks old) injected with MB cells
Dosage:	100 mg/kg
Administration:	Intraperitoneal injection; 5 days/week
Result:	Significantly extended the survival of MB xenografts in vivo.

性状

Solid

溶解性数据

In Vitro:

DMSO : 125 mg/mL (464.13 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	3.7131 mL	18.5653 mL	37.1306 mL
5 mM	0.7426 mL	3.7131 mL	7.4261 mL
10 mM	0.3713 mL	1.8565 mL	3.7131 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: ≥ 2.08 mg/mL (7.72 mM); Clear solution

此方案可获得 ≥ 2.08 mg/mL (7.72 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。
将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液
2.

请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in saline)
Solubility: ≥ 2.08 mg/mL (7.72 mM); Clear solution

此方案可获得 ≥ 2.08 mg/mL (7.72 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
3.

请依序添加每种溶剂： 10% DMSO 90% corn oil
Solubility: ≥ 2.08 mg/mL (7.72 mM); Clear solution

此方案可获得 ≥ 2.08 mg/mL (7.72 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。