Licochalcone D 甘草查尔酮 D|cas:144506-15-0|西域试剂

Licochalcone D 甘草查尔酮 D,99.81%

产品编号：Bellancom-N4187| CAS NO：144506-15-0| 分子式：C₂₁H₂₂O₅| 分子量：354.40

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货号	包装	价格	库存与货期	购买量	操作
Bellancom-N4187		￥1710.00	杭州北京（现货）
Bellancom-N4187		￥5140.00	杭州北京（现货）

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Licochalcone D 甘草查尔酮 D

产品介绍

Licochalcone D 是存在于 Glycyrrhiza uralensis 中的黄酮类化合物，是具有口服活性的 NF-κB p65 的有效抑制剂。Licochalcone D 具有抗氧化、抗炎、抗癌等作用。

生物活性

Licochalcone D, a flavonoid compound mainly existing in the root of Glycyrrhiza uralensis, is a potent and orally active inhibitor of NF-kappaB (NF-κB) p65. Licochalcone D possesses antioxidant, anti-inflammatory, anti-cancer properties.

体外研究

Licochalcone D (10 μM; 1 h) negatively regulates Lipopolysaccharides (LPS) (HY-D1056)-induced NF-κB activation by inhibiting the phosphorylation and transactivation of NF-κB p65, inhibits the LPS-induced activation of protein kinase A (PKA), and significantly inhibits the LPS-induced expression of TNFα and MCP-1.
Licochalcone D (10 μM; 24 h) significantly inhibits LPS-induced iNOS expression and NO production.
Licochalcone D (0-90 μM; 24 h) inhibits the proliferation of human melanoma cells, induces the apoptosis of A375 cells, decreases the mitochondrial membrane potential and increases ROS production in A375 cells.
Licochalcone D (0-25 μM; 24 h) decreases the migration and invasion ability of the A375 cells, decreased the protein levels of MMP-2 and MMP-9.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis

Cell Line:	RAW264.7 cells
Concentration:	10 μM
Incubation Time:	1 h or 24 h (iNOS), following LPS stimulation (1 μg/mL) for 30 min
Result:	Inhibited the phosphorylation of NF-κB at serine 276 but not at serine 536. Inhibited the LPS-induced expression of TNFα mRNA and MCP-1 mRNA. Strongly inhibited the expression of iNOS.

Cell Viability Assay

Cell Line:	A375 cells or SK-MEL-5
Concentration:	0, 1, 2.5, 5, 15, 30, 45, 60, 75 and 90 μM (A375) or 0, 20, 40, 60 and 80 μM (SK-MEL-5)
Incubation Time:	24 h
Result:	Inhibited the proliferation of A375 and SK-MEL-5 in a concentration dependent manner.

Apoptosis Analysis

Cell Line:	A375
Concentration:	0, 30, 60 and 90 μM
Incubation Time:	24 h
Result:	Cells exhibited obvious apoptotic characteristics after the treatment.

RT-PCR

Cell Line:	A375
Concentration:	0, 30, 60 and 90 μM
Incubation Time:	24 h
Result:	Downregulated the mRNA level of Bcl-2 and upregulated the mRNA levels of caspase-3, caspase-9 and Bax.

Cell Invasion Assay

Cell Line:	A375
Concentration:	25 μM
Incubation Time:	24 h
Result:	Significantly suppressed cell invasion.

Cell Migration Assay

Cell Line:	A375
Concentration:	25 μM
Incubation Time:	24 h
Result:	Significantly suppressed cell migration in the A375 cells.

体内研究
(In Vivo)

Licochalcone D (25 and 50 mg/kg; i.g.; daily for a week) inhibits the tumor growth in a mouse xenograft model of murine melanoma B16F0 cells.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	C57BL/6 mice, B16F0 tumor model
Dosage:	25 and 50 mg/kg
Administration:	Intragastric administration, daily for a week
Result:	Obviously lowered the tumor growth rates, the tumor growth inhibition rates were calculated to be 32.0 and 54.1% at 25 and 50 mg/kg, respectively.

体内研究

Licochalcone D (25 and 50 mg/kg; i.g.; daily for a week) inhibits the tumor growth in a mouse xenograft model of murine melanoma B16F0 cells.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	C57BL/6 mice, B16F0 tumor model
Dosage:	25 and 50 mg/kg
Administration:	Intragastric administration, daily for a week
Result:	Obviously lowered the tumor growth rates, the tumor growth inhibition rates were calculated to be 32.0 and 54.1% at 25 and 50 mg/kg, respectively.

体内研究

Licochalcone D (25 and 50 mg/kg; i.g.; daily for a week) inhibits the tumor growth in a mouse xenograft model of murine melanoma B16F0 cells.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	C57BL/6 mice, B16F0 tumor model
Dosage:	25 and 50 mg/kg
Administration:	Intragastric administration, daily for a week
Result:	Obviously lowered the tumor growth rates, the tumor growth inhibition rates were calculated to be 32.0 and 54.1% at 25 and 50 mg/kg, respectively.

性状

Solid

溶解性数据

In Vitro:

DMSO : 125 mg/mL (352.71 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	2.8217 mL	14.1084 mL	28.2167 mL
5 mM	0.5643 mL	2.8217 mL	5.6433 mL
10 mM	0.2822 mL	1.4108 mL	2.8217 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: ≥ 2.08 mg/mL (5.87 mM); Clear solution

此方案可获得 ≥ 2.08 mg/mL (5.87 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。
将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液
2.

请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in saline)
Solubility: ≥ 2.08 mg/mL (5.87 mM); Clear solution

此方案可获得 ≥ 2.08 mg/mL (5.87 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明