BI-6015|cas:93987-29-2|西域试剂

BI-6015,98.67%

产品编号：Bellancom-108469| CAS NO：93987-29-2| 分子式：C₁₅H₁₃N₃O₄S| 分子量：331.35

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货号	价格	库存与货期
Bellancom-108469	￥700.00	杭州北京（现货）
Bellancom-108469	￥1200.00	杭州北京（现货）
Bellancom-108469	￥2600.00	杭州北京（现货）
Bellancom-108469	￥4500.00	杭州北京（现货）
Bellancom-108469	￥7900.00	杭州北京（现货）

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BI-6015

产品介绍

BI-6015 是一种肝细胞核因子 4α (HNF4α) 拮抗剂，可抑制已知 HNF4α 靶基因的表达。BI6015 通过 HNF4α 拮抗作用抑制胰岛素启动子活性。BI-6015 可用于癌症和糖尿病的研究。

生物活性

BI-6015 is a hepatocyte nuclear factor 4α (HNF4α) antagonist that can inhibit the expression of known HNF4α target genes. BI6015 represses insulin promoter activity through HNF4α antagonism. BI-6015 can be used for the research of cancer and diabetes.

体外研究

BI-6015 (1.25-20 μM; 24-72 h) is cytotoxic to human hepatocellular carcinoma (HCC).
BI-6015 (2.5-10 μM; 5-48 h) inhibits HNF4α gene expression in HepG2 cells.
BI-6015 (5 μM; 3 d) induces hepatic steatosis in primary murine hepatocytes.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay

Cell Line:	Hep3B-Luc cells and primary hepatocytes
Concentration:	1.25, 2.5, 5, 10, 20 μM
Incubation Time:	24, 48, 72 hours
Result:	Was markedly toxic to Hep3B cells but spared primary hepatocytes.

体内研究
(In Vivo)

BI-6015 (10-30 mg/kg; i.p. once daily for 5 days) induces loss of HNF4α expression and hepatic steatosis in mice.
BI-6015 (10-30 mg/kg; i.p. daily or every other day for 20-57 days) induces apoptosis in a human hepatocellular carcinoma mouse model.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

性状

Solid

溶解性数据

In Vitro:

DMSO : 50 mg/mL (150.90 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	3.0180 mL	15.0898 mL	30.1796 mL
5 mM	0.6036 mL	3.0180 mL	6.0359 mL
10 mM	0.3018 mL	1.5090 mL	3.0180 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： 10% DMSO 90% corn oil
Solubility: ≥ 2.5 mg/mL (7.54 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (7.54 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。