帕博利珠单抗|Pembrolizumab;MK-3475|cas:1374853-91-4|西域试剂

帕博利珠单抗,Pembrolizumab;MK-3475,99%

产品编号：西域试剂-P9902| CAS NO：1374853-91-4| 分子量：146266.23

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货号	价格	库存与货期
西域试剂-P9902	￥2900.00	杭州北京（现货）
西域试剂-P9902	￥5900.00	杭州北京（现货）
西域试剂-P9902	￥7600.00	杭州北京（现货）

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产品介绍

Pembrolizumab (MK-3475) 是一种人源化 IgG4 抗体，为 PD-1 的抑制剂，用于癌症免疫的研究。

生物活性

Pembrolizumab (MK-3475) is a humanized IgG4 antibody inhibiting the programmed cell death 1 (PD-1) receptor, used in cancer immunotherapy.

体外研究

Pembrolizumab (MK-3475) increases the secretion of cytokines IFN-γ, TNF-α and the apoptotic cell death of A549 cells.
Pembrolizumab improves the αROR1-CAR T-mediated cytotoxicity, and reduces tumorigenesis in the co-cultured αROR1-CAR T and A549 cells by blocking PD-1::PD-L1 interaction in αROR1-CAR T cells.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cytotoxicity Assay

Cell Line:	αROR1-CAR T and A549 cells
Concentration:	10 μg/mL
Incubation Time:	Adding Pembrolizumab after A549-Red-Fluc cells preconditioned in elevated pressure (+ 100 mmHg) for 7 days.
Result:	Enhanced αROR1‑CAR T killing of A549 lung cancer cells under elevated pressure.

Apoptosis Analysis

Cell Line:	A549 cells
Concentration:	10 μg/mL
Incubation Time:
Result:	Increased the apoptotic cell death in A549 cells, and improve the αROR1-CAR T-mediated cytotoxicity.

体内研究
(In Vivo)

Pembrolizumab inhibits tumor size in A549 xenograft mice pretreated by pressure.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Six-week-old male BALB/c athymic nude mice
Dosage:	5 mg/kg
Administration:	Pembrolizumab (5 mg/kg; Intravenous injection; Once)
Result:	Reduced tumorigenesis in αROR1-CAR T cells and co-cultured with A549 cells.

体内研究

Pembrolizumab inhibits tumor size in A549 xenograft mice pretreated by pressure.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Six-week-old male BALB/c athymic nude mice
Dosage:	5 mg/kg
Administration:	Pembrolizumab (5 mg/kg; Intravenous injection; Once)
Result:	Reduced tumorigenesis in αROR1-CAR T cells and co-cultured with A549 cells.

体内研究

Pembrolizumab inhibits tumor size in A549 xenograft mice pretreated by pressure.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Six-week-old male BALB/c athymic nude mice
Dosage:	5 mg/kg
Administration:	Pembrolizumab (5 mg/kg; Intravenous injection; Once)
Result:	Reduced tumorigenesis in αROR1-CAR T cells and co-cultured with A549 cells.

性状

Liquid

溶解性数据

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Please store the product under the recommended conditions in the Certificate of Analysis.

参考文献

. Schachter J, et al. Pembrolizumab versus ipilimumab for advanced melanoma: final overall survival results of a multicentre, randomised, open-label phase 3 study (KEYNOTE-006). Lancet. 2017 Aug 16. pii: S0140-6736(17)31601-X. [Content Brief]

. Zhenglin Ou, et al. Pressure increases PD‑L1 expression in A549 lung adenocarcinoma cells and causes resistance to anti‑ROR1 CAR T cell‑mediated cytotoxicity, Sci Rep [Content Brief]

. Amita Patnaik, et al. Phase I Study of Pembrolizumab (MK-3475; Anti–PD-1 Monoclonal Antibody) in Patients with Advanced Solid Tumors. Clin Cancer Res.2015Oct1;21(19):4286-93.

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