Coppersensor 1|cas:874748-20-6|西域试剂

Coppersensor 1,98.0%

产品编号：Bellancom-141511| CAS NO：874748-20-6| 分子式：C₃₀H₅₀BF₂N₃S₄| 分子量：629.81

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货号	价格	库存与货期
Bellancom-141511	￥1100.00	杭州北京（现货）
Bellancom-141511	￥2800.00	杭州北京（现货）
Bellancom-141511	￥4200.00	杭州北京（现货）

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Coppersensor 1

产品介绍

Coppersensor-1 (CS1) 是一种膜透性荧光染料。Coppersensor-1 对 Cu⁺ 具有亲和力，其亲和力在皮摩尔范围，对竞争细胞金属离子具有很高的选择性。Coppersensor-1 作为探针，可以选择性、灵敏地检测包括活细胞在内的生物样品中的铜 (I) 离子 (Cu⁺)。Coppersensor-1 可用于癌症、心血管疾病、神经系统疾病等严重疾病的成像研究。储存方式：避光。

生物活性

Coppersensor-1 (CS1) is a membrane-permeable fluorescent dye. Coppersensor-1 has a picomolar affinity for Cu⁺ with high selectivity over competing cellular metalions. Coppersensor-1 as a probe, can selective and sensitive detection of copper(I) ions (Cu⁺) in biological samples, including live cells. Coppersensor-1 can be used for the research of imaging of severe diseases such as cancer, cardiovascular disorders and neurogenerative diseases. Storage: protect from light.

体外研究

Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
A. Preparation of reagent stock solutions for imaging:
1. Prepare a 1 mM stock solution of CS1 (MW 630 g/moL) in DMSO by dissolving 0.63 mg of solid CS1 per milliliter of DMSO solvent.
2. Prepare a 10 mM stock solution of CuCl₂ (MW 134 g/moL) by dissolving 1.34 mg of solid CuCl₂ per milliliter of Millipore water solvent.
B. CS1 labeling of live cells:
1. Imaging experiments with cells will require 1-2 d with cells in culture. Incubate the cells according to your normal protocol.
2. One day before imaging, cells are passaged and plated on 18-mm glass coverslips coated with poly-L-lysine (50 μg/mL). Adherent cells for imaging are grown to 50-80% confluency.
3. Remove cells from incubator, and transfer one coverslip into a 35-mm Petri dish containing 3 mL PBS buffer.
4. To the Petri dish, add 15 μL of a 1 mM CS1 stock solution to give a final dye concentration of 5 μM. Mix thoroughly.
(Higher concentrations of dye may result in high levels of background fluorescence)
5. Incubate for 5-20 min in the dark at 25 or 37℃.
C. Imaging the CS1-labeled cells:
1. Imageing in standard confocal microscope with 543 nm excitation.
(CS1 can be imaged using any type of fluorescence microscope, including epifluorescence, confocal and multiphoton)

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究

性状

Oil

溶解性数据

In Vitro:

DMSO : 25 mg/mL (39.69 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	1.5878 mL	7.9389 mL	15.8778 mL
5 mM	0.3176 mL	1.5878 mL	3.1756 mL
10 mM	0.1588 mL	0.7939 mL	1.5878 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式