CITCO|cas:338404-52-7|西域试剂

CITCO,99.17%

产品编号：Bellancom-103244| CAS NO：338404-52-7| 分子式：C₁₉H₁₂Cl₃N₃OS| 分子量：436.74

本网站销售的所有产品仅用于工业应用或者科学研究等非医疗目的,不可用于人类或动物的临床诊断或者治疗,非药用,非食用，

货号	价格	库存与货期
Bellancom-103244	￥700.00	杭州北京（现货）
Bellancom-103244	￥1200.00	杭州北京（现货）
Bellancom-103244	￥2500.00	杭州北京（现货）
Bellancom-103244	￥4000.00	杭州北京（现货）

增值税发票√顺丰快递√订货电话：18601927057

CITCO

产品介绍

CITCO 是一种组成型雄甾烷 (Constitutive androstane) 受体激动剂，抑制脑肿瘤干细胞 (BTSCs) 的生长和扩增。CITCO 是一种咪唑并噻唑衍生物，其作为人 CAR 的选择性激动剂起作用。CITCO 对于孕烷 X 受体 (PXR) 的 EC₅₀ 为 49 nM，并且对其他核受体没有活性。

生物活性

CITCO, an imidazothiazole derivative, is a selective Constitutive androstane receptor (CAR) agonist. CITCO inhibits growth and expansion of brain tumour stem cells (BTSCs) and has an EC₅₀ of 49 nM over pregnane X receptor (PXR), and no activity on other nuclear receptors.

体外研究

CITCO (1-50 μM; 48 hours) results in a dose-dependent inhibition of viable cell count and proliferation in both T98G and U87MG glioma and BTSCs.
CITCO (2.5, 5 μM; 48 hours) induces cell cycle arrest differentially in different BTSCs in culture, but not in normal astrocytes.
CITCO (2.5-10 μM; 48 hours) induces apoptosis in BTSCs in culture in dose dependently, but not in normal astrocytes.
CITCO (0-25 μM; 48 hours) causes the T98G and U87MG glioma and BTSCs expressing very low levels of CAR protein that increased significantly.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay

Cell Line:	T98G, U87MG, DB29 and DB33 human glioma cells, astrocytes
Concentration:	1, 2.5, 5, 10, 25, 50 μM
Incubation Time:	48 hours
Result:	Resulted in a dose-dependent inhibition of viable cell count and proliferation.

Cell Cycle Analysis

Cell Line:	The T98G, U87MG, DB29 and DB33 glioma cells
Concentration:	2.5, 5 μM
Incubation Time:	48 hours
Result:	Induced cell cycle arrest differentially in different BTSCs in culture.

Apoptosis Analysis

Cell Line:	The T98G, U87MG, DB29 and DB33 glioma cells
Concentration:	2.5, 5 or 10 μM
Incubation Time:	48 hours
Result:	Increased the levels of Annexin V-positive apoptotic cells in dose dependently.

Western Blot Analysis

Cell Line:	T98G, U87MG, DB29 and DB33 glioma cells
Concentration:	0 to 25 μM
Incubation Time:	48 hours
Result:	The T98G, U87MG glioma and BTSCs expressed very low levels of CAR protein that increased significantly.

体内研究
(In Vivo)

CITCO (intraperitoneal; on days 22, 24, 26, 30 and 36) with 25 μg results a significant decrease in tumour growth, which further decreases to an undetectable level after treatment with 100 μg CITCO .

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Six- to eight-week-old male athymic nude mice
Dosage:	25 or 100 μg
Administration:	Intraperitoneal; on days 22, 24, 26, 30 and 36
Result:	Decreased tumour growth.

体内研究

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Six- to eight-week-old male athymic nude mice
Dosage:	25 or 100 μg
Administration:	Intraperitoneal; on days 22, 24, 26, 30 and 36
Result:	Decreased tumour growth.

体内研究

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Six- to eight-week-old male athymic nude mice
Dosage:	25 or 100 μg
Administration:	Intraperitoneal; on days 22, 24, 26, 30 and 36
Result:	Decreased tumour growth.

性状

Solid

溶解性数据

In Vitro:

DMSO : 25 mg/mL (57.24 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	2.2897 mL	11.4485 mL	22.8969 mL
5 mM	0.4579 mL	2.2897 mL	4.5794 mL
10 mM	0.2290 mL	1.1448 mL	2.2897 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

参考文献