BML-210|cas:537034-17-6|西域试剂

BML-210,98.06%

产品编号：Bellancom-19350| CAS NO：537034-17-6| 分子式：C₂₀H₂₅N₃O₂| 分子量：339.43

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货号	价格	库存与货期
Bellancom-19350	￥600.00	杭州北京（现货）
Bellancom-19350	￥900.00	杭州北京（现货）
Bellancom-19350	￥2500.00	杭州北京（现货）
Bellancom-19350	￥4500.00	杭州北京（现货）

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BML-210

产品介绍

BML-210 是一种有效的 HDAC 抑制剂。BML-210 可以抑制 HDAC4-VP16 驱动的报告信号，IC₅₀ 为 ~5 µM。BML-210 对 HDAC4:MEF2 交互具有特定的破坏性影响。BML-210 导致 G0/G1 期增加。BML-210 诱导细胞凋亡，并在小鼠原位乳腺肿瘤中显示出抗肿瘤活性。

生物活性

BML-210 is a potent HDAC inhibitor. BML-210 can inhibit the HDAC4-VP16-driven reporter signal with an apparent IC₅₀ of ∼5 µM. BML-210 has a specific disruptive effect on the HDAC4:MEF2 interaction. BML-210 causes an increase in the G0/G1 phase. BML-210 induces apoptosis and displays antitumour activities in orthotopic mammary tumours in mice.

体外研究

BML-210 (10, 20 μM; 24, 48小时) 抑制 NB4 细胞的增殖和生长。
BML-210 (10, 20 μM; 24, 48小时) 导致 NB4 细胞在 S 期的比例降低，G0/G1 期的比例增加。
BML-210 (10, 20 μM; 24, 48小时) 在 20μM 时对NB4细胞产生细胞毒性作用。BML-210 以 10μM 剂量可以诱导凋亡细胞死亡。
BML-210 (10, 20 μM; 24, 48小时) 抑制 NB4 细胞中 HDAC 的表达和活性。
BML-210 不降低 HDAC4-VP16 的表达。

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay

Cell Line:	NB4 cells
Concentration:	10, 20 μM
Incubation Time:	24, 48 hours
Result:	Inhibited cell proliferation and growth inhibition of NB4 cells in a dose- and time-dependent manner.

Cell Cycle Analysis

Cell Line:	NB4 cells
Concentration:	10, 20 μM
Incubation Time:	24, 48 hours
Result:	Caused a decrease in the proportion of NB4 cells in the S phase and an increase in the G0/G1 phase. Caused an increase in the G0/G1 phase up to 70% at 24 and 48 h with 10 μM.

Cell Cytotoxicity Assay

Cell Line:	NB4 cells
Concentration:	10, 20 μM
Incubation Time:	24, 48 hours
Result:	Caused cytotoxic effects on NB4 cells in a dose- and time-dependent manner.

Apoptosis Analysis

Cell Line:	NB4 cells
Concentration:	10, 20 μM
Incubation Time:	24, 48 hours
Result:	At a dose of 10 μM induced apoptotic cell death.

Western Blot Analysis

Cell Line:	NB4 cells
Concentration:	10, 20 μM
Incubation Time:	24, 48 hours
Result:	At 10 μM dose inhibited HDAC1 gene expression up to 36% after 48 h of treatment Inhibited HDAC expression up to 74% at 8 h point at 20 μM. Had very low effect on HDAC 2 and HDAC 3 expression.

体内研究
(In Vivo)

BML-210 (20 mg/kg; 腹腔给药; 每周 3 次，持续两周) 显着抑制肿瘤生长和重量。BML-210 对免疫缺陷裸鼠 (Nu/J) 的肿瘤生长和体重没有影响。

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Female C57BL/6 mice with mouse breast cancer EO771 cells
Dosage:	20 mg/kg
Administration:	IP; three times per week for two weeks
Result:	Notably suppressed the tumour growth and weight.

体内研究

BML-210 (20 mg/kg; 腹腔给药; 每周 3 次，持续两周) 显着抑制肿瘤生长和重量。BML-210 对免疫缺陷裸鼠 (Nu/J) 的肿瘤生长和体重没有影响。

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Female C57BL/6 mice with mouse breast cancer EO771 cells
Dosage:	20 mg/kg
Administration:	IP; three times per week for two weeks
Result:	Notably suppressed the tumour growth and weight.

体内研究

BML-210 (20 mg/kg; 腹腔给药; 每周 3 次，持续两周) 显着抑制肿瘤生长和重量。BML-210 对免疫缺陷裸鼠 (Nu/J) 的肿瘤生长和体重没有影响。

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Female C57BL/6 mice with mouse breast cancer EO771 cells
Dosage:	20 mg/kg
Administration:	IP; three times per week for two weeks
Result:	Notably suppressed the tumour growth and weight.

性状

Solid

溶解性数据

In Vitro:

DMSO : ≥ 30 mg/mL (88.38 mM)

* "≥" means soluble, but saturation unknown.

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	2.9461 mL	14.7306 mL	29.4612 mL
5 mM	0.5892 mL	2.9461 mL	5.8922 mL
10 mM	0.2946 mL	1.4731 mL	2.9461 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： 10% DMSO 90% corn oil
Solubility: ≥ 2.5 mg/mL (7.37 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (7.37 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。