J14|cas:1043854-13-2|西域试剂

J14,99.06%

产品编号：Bellancom-135008| CAS NO：1043854-13-2| 分子式：C₂₈H₂₅ClN₄O₂S| 分子量：517.04

本网站销售的所有产品仅用于工业应用或者科学研究等非医疗目的,不可用于人类或动物的临床诊断或者治疗,非药用,非食用，

货号	价格	库存与货期
Bellancom-135008	￥500.00	杭州北京（现货）
Bellancom-135008	￥850.00	杭州北京（现货）
Bellancom-135008	￥1250.00	杭州北京（现货）
Bellancom-135008	￥1950.00	杭州北京（现货）
Bellancom-135008	￥2950.00	杭州北京（现货）

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J14

产品介绍

J14 是一种可逆 sulfiredoxin 抑制剂，IC₅₀ 为 8.1 μM。J14 通过抑制 sulfiredoxin 诱导氧化应激 (导致细胞内 ROS 积累)，从而导致细胞毒性和癌细胞死亡。

生物活性

J14 is a reversible sulfiredoxin inhibitor with an IC₅₀ of 8.1 μM. J14 induces oxidative stress (intracellular ROS accumulation) by inhibiting sulfiredoxin, leading to cytotoxicity and cancer cell death.

体外研究

J14 (0-100 μM; 0-96 hours; A549 cells) treatment inhibits the growth of A549 cells in a concentration- and a time- dependent manner, and its half inhibitory concentration for the growth of A549 cells was 15.7 μM.
J14 (20 μM; 48-72 hours; A549 cells) treatment causes not only the release of cytochrome c into the cytosol, but also the activation of caspase-3 and caspase-9. J14 induces oxidative damage to mitochondria, resulting in caspase-mediated apoptosis.
J14 treatment significantly increases the accumulation of sulfinic peroxiredoxins and intracellular ROS. Excess accumulation of intracellular ROS causes oxidative damage, leading to cell death. J14 significantly induces cell death in A549 cells in a time-dependent manner, resulting in approximately 40% cell death in 96 hours.
J14 induces oxidative mitochondrial damage and apoptosis.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay

Cell Line:	A549 cells
Concentration:	0-100 μM
Incubation Time:	0 hour, 24 hours, 48 hours, 72 hours, 96 hours
Result:	Inhibited the growth of A549 cells in a concentration- and a time- dependent manner.

Western Blot Analysis

Cell Line:	A549 cells
Concentration:	20 μM
Incubation Time:	48 hours, 72 hours
Result:	Caused not only the release of cytochrome c into the cytosol, but also the activation of caspase-3 and caspase-9.

体内研究
(In Vivo)

J14 (50 mg/kg; intraperitoneal injection; daily; for 16 days; BALB/c nude female mice) treatment significantly reduces the average tumor volume. The masses and weights of the primary tumors excised from the J14-treated mice are significantly lower compared with those of the control mice.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Six-week-old BALB/c nude female mice injected with A549 cells
Dosage:	50 mg/kg
Administration:	Intraperitoneal injection; daily; for 16 days
Result:	Significantly reduced the growth of human lung tumor without acute toxicity.

体内研究

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Six-week-old BALB/c nude female mice injected with A549 cells
Dosage:	50 mg/kg
Administration:	Intraperitoneal injection; daily; for 16 days
Result:	Significantly reduced the growth of human lung tumor without acute toxicity.

体内研究

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Six-week-old BALB/c nude female mice injected with A549 cells
Dosage:	50 mg/kg
Administration:	Intraperitoneal injection; daily; for 16 days
Result:	Significantly reduced the growth of human lung tumor without acute toxicity.

性状

Solid

溶解性数据

In Vitro:

DMSO : ≥ 125 mg/mL (241.76 mM)

* "≥" means soluble, but saturation unknown.

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	1.9341 mL	9.6704 mL	19.3409 mL
5 mM	0.3868 mL	1.9341 mL	3.8682 mL
10 mM	0.1934 mL	0.9670 mL	1.9341 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: ≥ 2.17 mg/mL (4.20 mM); Clear solution

此方案可获得 ≥ 2.17 mg/mL (4.20 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 21.7 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。
将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液
2.

请依序添加每种溶剂： 10% DMSO 90% corn oil
Solubility: ≥ 2.17 mg/mL (4.20 mM); Clear solution

此方案可获得 ≥ 2.17 mg/mL (4.20 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。
以 1 mL 工作液为例，取 100 μL 21.7 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。