Z-LEHD-FMK|cas:210345-04-3|西域试剂

Z-LEHD-FMK,98.0%

产品编号：Bellancom-P1010| CAS NO：210345-04-3| 分子式：C₃₂H₄₃FN₆O₁₀| 分子量：690.72

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货号	包装	价格	库存与货期	购买量	操作
Bellancom-P1010		￥4200.00	杭州北京（现货）

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Z-LEHD-FMK

产品介绍

Z-LEHD-FMK 是一种选择性和不可逆的 caspase-9 抑制剂，可防止致命的再灌注损伤并减弱细胞凋亡。Z-LEHD-FMK 在大鼠脊髓损伤模型中也表现出神经保护作用。

生物活性

Z-LEHD-FMK is a selective and irreversible inhibitor of caspase-9, protects against lethal reperfusion injury and attenuates apoptosis. Z-LEHD-FMK exhibits the neuroprotective effect in a rat model of spinal cord trauma.

体外研究

Z-LEHD-FMK (20 μM; pretreated for 30 min) completely protects HCT116 and 293 cells from TRAIL-induced toxicity.
Z-LEHD-FMK (20 μM ; 6 h) protects normal human hepatocytes from TRAIL-induced apoptosis.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Apoptosis Analysis

Cell Line:	SW480, H460, HCT116 and 293 cells
Concentration:	20 μM
Incubation Time:	Pretreated for 30 min
Result:	Protected HCT116 and 293 cells from TRAIL-induced apoptosis.

Western Blot Analysis

Cell Line:	HCT116, SW480 cells
Concentration:	20 μM
Incubation Time:	2 h
Result:	Protected procaspase 3 from cleavage in HCT116 cells but not in SW480 cells, especially at the 16-h time point.

体内研究
(In Vivo)

Z-LEHD-FMK (0.8 μmol/kg; i.v. for 7 d) protects neurons, glia, myelin, axons, and intracellular organelles in spinal cord injury (SCI) rats.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male Wistar albino rats (250-350 g) with SCI
Dosage:	0.8 μmol/kg
Administration:	I.v. for 1 or 7 days
Result:	Decreased the mean apoptotic cell count at 24 hours and 7 days postinjury.

体内研究

Z-LEHD-FMK (0.8 μmol/kg; i.v. for 7 d) protects neurons, glia, myelin, axons, and intracellular organelles in spinal cord injury (SCI) rats.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male Wistar albino rats (250-350 g) with SCI
Dosage:	0.8 μmol/kg
Administration:	I.v. for 1 or 7 days
Result:	Decreased the mean apoptotic cell count at 24 hours and 7 days postinjury.

体内研究

Z-LEHD-FMK (0.8 μmol/kg; i.v. for 7 d) protects neurons, glia, myelin, axons, and intracellular organelles in spinal cord injury (SCI) rats.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male Wistar albino rats (250-350 g) with SCI
Dosage:	0.8 μmol/kg
Administration:	I.v. for 1 or 7 days
Result:	Decreased the mean apoptotic cell count at 24 hours and 7 days postinjury.

性状

Solid

溶解性数据

In Vitro:

DMSO : 100 mg/mL (144.78 mM; Need ultrasonic and warming)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	1.4478 mL	7.2388 mL	14.4776 mL
5 mM	0.2896 mL	1.4478 mL	2.8955 mL
10 mM	0.1448 mL	0.7239 mL	1.4478 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

Sealed storage, away from moisture