Hydroxy-α-sanshool 羟基-α-山椒素|cas:83883-10-7|西域试剂

Hydroxy-α-sanshool 羟基-α-山椒素,99.13%

产品编号：Bellancom-N6825| CAS NO：83883-10-7| 分子式：C₁₆H₂₅NO₂| 分子量：263.38

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货号	包装	价格	库存与货期	购买量	操作
Bellancom-N6825		￥2950.00	杭州北京（现货）
Bellancom-N6825		￥4800.00	杭州北京（现货）

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Hydroxy-α-sanshool 羟基-α-山椒素

产品介绍

Hydroxy-α-sanshool 是 TRPA1 和 TRPV1 激动剂，EC₅₀ 分别为 69 和 1.1 µM，可用于疼痛的研究。

生物活性

Hydroxy-α-sanshool is a transient receptor potential ankyrin 1 (TRPA1) and TRP vanilloid 1 (TRPV1) agonist with EC₅₀s of 69 and 1.1 µM, respectively. Hydroxy-α-sanshool can be used for pain research.

体外研究

Hydroxy-α-sanshool (0-10 mM; 20 s) induces inward currents in cultured DRG neuron.
Hydroxy-α-sanshool (1 mM; 0-1 min) increases intracellular Ca²⁺ in cultured DRG neuron.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay

Cell Line:	Dorsal root ganglion (DRG) neurons
Concentration:	0-10 mM
Incubation Time:	20 s
Result:	Induced small but robust inward currents for DRG neuron with an EC₅₀ of 66.2 μM.

Cell Viability Assay

Cell Line:	Dorsal root ganglion (DRG) neurons
Concentration:	1 mM
Incubation Time:	0-1 min
Result:	Led to an intracellular Ca²⁺ increase in the majority of DRG neurons tested (98 of 100).

体内研究
(In Vivo)

Hydroxy-α-sanshool (intradermal injection; 4 mg per mouse; once) shows paw-licking response in wild-type and TRPV1-deficient mice.
Hydroxy-α-sanshool (1 mM) is markedly aversive to WT mice, but in TRPV1 KO mice, the aversion is almost entirely eliminated (P<0.001).

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Wild-type and TRPV1-deficient mice
Dosage:	4 mg per mouse
Administration:	Intradermal injection; 4 mg per mouse; once
Result:	Reduced paw-licking response in TRPV1-deficient mice (160 times, P < 0.05). Showed paw-licking response in wild-type mice (mean 284.8 times) in 20 min.

体内研究

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Wild-type and TRPV1-deficient mice
Dosage:	4 mg per mouse
Administration:	Intradermal injection; 4 mg per mouse; once
Result:	Reduced paw-licking response in TRPV1-deficient mice (160 times, P < 0.05). Showed paw-licking response in wild-type mice (mean 284.8 times) in 20 min.

体内研究

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Wild-type and TRPV1-deficient mice
Dosage:	4 mg per mouse
Administration:	Intradermal injection; 4 mg per mouse; once
Result:	Reduced paw-licking response in TRPV1-deficient mice (160 times, P < 0.05). Showed paw-licking response in wild-type mice (mean 284.8 times) in 20 min.

性状

Viscous liquid

溶解性数据

In Vitro:

DMSO : 100 mg/mL (379.68 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	3.7968 mL	18.9840 mL	37.9680 mL
5 mM	0.7594 mL	3.7968 mL	7.5936 mL
10 mM	0.3797 mL	1.8984 mL	3.7968 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: 2.5 mg/mL (9.49 mM); Suspended solution; Need ultrasonic

此方案可获得 2.5 mg/mL (9.49 mM) 的均匀悬浊液，悬浊液可用于口服和腹腔注射。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。
将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液
2.

请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in saline)
Solubility: 2.5 mg/mL (9.49 mM); Suspended solution; Need ultrasonic

此方案可获得 2.5 mg/mL (9.49 mM) 的均匀悬浊液，悬浊液可用于口服和腹腔注射。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
3.

请依序添加每种溶剂： 10% DMSO 90% corn oil
Solubility: ≥ 2.08 mg/mL (7.90 mM); Clear solution

此方案可获得 ≥ 2.08 mg/mL (7.90 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。