ESI-05 NSC 116966|cas:5184-64-5|西域试剂

ESI-05 NSC 116966,99.94%

产品编号：Bellancom-117656| CAS NO：5184-64-5| 分子式：C₁₆H₁₈O₂S| 分子量：274.38

本网站销售的所有产品仅用于工业应用或者科学研究等非医疗目的,不可用于人类或动物的临床诊断或者治疗,非药用,非食用，

货号	价格	库存与货期
Bellancom-117656	￥600.00	杭州北京（现货）
Bellancom-117656	￥1050.00	杭州北京（现货）
Bellancom-117656	￥3200.00	杭州北京（现货）
Bellancom-117656	￥5500.00	杭州北京（现货）

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ESI-05 NSC 116966

产品介绍

ESI-05 是一种特异性 EPAC2 抑制剂。ESI-05 抑制 cAMP 介导的 EPAC2 GEF 活性，IC₅₀ 值为 0.43 μM。ESI-05 可用于糖尿病、胰岛素分泌和神经系统疾病的研究。

生物活性

ESI-05 is a specific exchange proteins directly activated by cAMP 2 (EPAC2) inhibitor. ESI-05 inhibits cAMP-mediated EPAC2 GEF activity with an IC₅₀ of 0.43 μM. ESI-05 can be used for the research of diabetes, insulin secretion and neurological disorders.

体外研究

ESI-05 (0.01 μM -1 nM) inhibits cAMP-mediated EPAC2 GEF activity with IC₅₀ of 0.43 μM, but completely ineffective in suppressing EPAC1 GEF activity.
ESI-05 (1, 5, 10, and 25 μM; 5 min) selectively modulates EPAC2 activation in living cells.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis

Cell Line:	HEK293 cells
Concentration:	1, 5, 10, and 25 μM
Incubation Time:	5 min
Result:	Led to a dose-dependent reduction of the EPAC-selective cAMP analog (007-AM) induced Rap1 activation.

体内研究
(In Vivo)

ESI-05 (2, 4, and 8 mg/kg) decreases neuronal apoptosis by inhibiting the p38/BIM pathway in vivo.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Intracranial hemorrhage (ICH) model
Dosage:	2, 4, and 8 mg/kg
Administration:
Result:	Decreased the apoptosis rate of nerve cells in the cortex accompanied by a corresponding decrease in the protein expression of phosphorylated p38, Bcl-2like protein 11 (BIM), and caspase-3.

体内研究

ESI-05 (2, 4, and 8 mg/kg) decreases neuronal apoptosis by inhibiting the p38/BIM pathway in vivo.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Intracranial hemorrhage (ICH) model
Dosage:	2, 4, and 8 mg/kg
Administration:
Result:	Decreased the apoptosis rate of nerve cells in the cortex accompanied by a corresponding decrease in the protein expression of phosphorylated p38, Bcl-2like protein 11 (BIM), and caspase-3.

体内研究

ESI-05 (2, 4, and 8 mg/kg) decreases neuronal apoptosis by inhibiting the p38/BIM pathway in vivo.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Intracranial hemorrhage (ICH) model
Dosage:	2, 4, and 8 mg/kg
Administration:
Result:	Decreased the apoptosis rate of nerve cells in the cortex accompanied by a corresponding decrease in the protein expression of phosphorylated p38, Bcl-2like protein 11 (BIM), and caspase-3.

性状

Solid

溶解性数据

In Vitro:

DMSO : 50 mg/mL (182.23 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	3.6446 mL	18.2229 mL	36.4458 mL
5 mM	0.7289 mL	3.6446 mL	7.2892 mL
10 mM	0.3645 mL	1.8223 mL	3.6446 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： 10% DMSO 90% corn oil
Solubility: ≥ 2.5 mg/mL (9.11 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (9.11 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。