LEI-401|cas:2393840-15-6|西域试剂

LEI-401,99.79%

产品编号：Bellancom-131181| CAS NO：2393840-15-6| 分子式：C₂₄H₃₁N₅O₂| 分子量：421.54

本网站销售的所有产品仅用于工业应用或者科学研究等非医疗目的,不可用于人类或动物的临床诊断或者治疗,非药用,非食用，

货号	价格	库存与货期
Bellancom-131181	￥1500.00	杭州北京（现货）
Bellancom-131181	￥2200.00	杭州北京（现货）
Bellancom-131181	￥4500.00	杭州北京（现货）
Bellancom-131181	￥7700.00	杭州北京（现货）
Bellancom-131181	￥13500.00	杭州北京（现货）

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LEI-401

产品介绍

LEI-401 是一种首创的、选择性的、通过血脑屏障的 NAPE-PLD (N-酰基磷酰乙醇胺磷脂酶 D) 抑制剂，IC₅₀ 为 27 nM。LEI-401 对小鼠情绪行为的调节。

生物活性

LEI-401 is a first-in-class, selective, and CNS-active NAPE-PLD (N-acylphosphatidylethanolamine phospholipase D) inhibitor, with an IC₅₀ of 27 nM. LEI-401 modulates emotional behavior in mice.

体外研究

LEI-401 reduced a broad range of NAEs including anandamide in neuronal cells in a NAPE-PLD-dependent manner. LEI-401 (0.04-20 μM; 30 minutes) dose-dependently reduces the labeling of NAPE-PLD with an IC₅₀ of 0.86 μM in hNAPE-PLD-transfected HEK293T cells. LEI-401 reduces NAE levels in Neuro-2a cells, but not in NAPE-PLD KO cells.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究

LEI-401 (30 mg/kg; i.p.) diminishes fear extinction in mice.
LEI-401 also activates HPA axis signaling.
LEI-401 (10 mg/kg; p.o.) treatment shows the t_1/2, C_max, t_max, AUC_last, and F values of 2.5 hours, 1370 ng/mL, 2 hours, 6760 h*ng/mL, and 25%, respectively.
LEI-401 (30 mg/kg; i.p.) treatment show the C_max, t_max, AUC_last, and F values of 10300 ng/mL, 1 hour, 38600 h*ng/mL, and 48%, respectively.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male 7–12-week-old C57BL/6J mice
Dosage:	30 mg/kg
Administration:	I.p.
Result:	Produced a significant increase in freezing as compared to vehicle.

Animal Model:	C57BL/6J mice
Dosage:	10 mg/kg
Administration:	P.o. (Pharmacokinetic Analysis)
Result:	The t_1/2, C_max, t_max, AUC_last, and F values were 2.5 hours, 1370 ng/mL, 2 hours, 6760 h*ng/mL, and 25%, respectively.

体内研究

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male 7–12-week-old C57BL/6J mice
Dosage:	30 mg/kg
Administration:	I.p.
Result:	Produced a significant increase in freezing as compared to vehicle.

Animal Model:	C57BL/6J mice
Dosage:	10 mg/kg
Administration:	P.o. (Pharmacokinetic Analysis)
Result:	The t_1/2, C_max, t_max, AUC_last, and F values were 2.5 hours, 1370 ng/mL, 2 hours, 6760 h*ng/mL, and 25%, respectively.

性状

Solid

溶解性数据

In Vitro:

DMSO : 100 mg/mL (237.23 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	2.3723 mL	11.8613 mL	23.7225 mL
5 mM	0.4745 mL	2.3723 mL	4.7445 mL
10 mM	0.2372 mL	1.1861 mL	2.3723 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： 10% DMSO 90% corn oil
Solubility: ≥ 2.5 mg/mL (5.93 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (5.93 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。