Epiberberine chloride 表小檗碱氯化物|cas:889665-86-5|西域试剂

Epiberberine chloride 表小檗碱氯化物,99.03%

产品编号：Bellancom-N0226A| CAS NO：889665-86-5| 分子式：C₂₀H₁₈ClNO₄| 分子量：371.81

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货号	价格	库存与货期
Bellancom-N0226A	￥900.00	杭州北京（现货）
Bellancom-N0226A	￥1200.00	杭州北京（现货）
Bellancom-N0226A	￥4400.00	杭州北京（现货）

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Epiberberine chloride 表小檗碱氯化物

产品介绍

Epiberberine chloride 是从黄连中得到的生物碱，为有效的 AChE 和 BChE 抑制剂，和非竞争性的 BACE1 抑制剂，IC₅₀ 值分别为 1.07，6.03 和 8.55 μM。Epiberberine chloride 具有抗氧化作用，能够清除 ONOO^- (IC₅₀，16.83 μM)，可用于阿尔滋海默症和糖尿病的研究。在 3T3-L1 细胞分化早期，Epiberberine 能够下调 Raf/MEK1/2/ERK1/2 和 AMPKα/Akt 信号通路。

生物活性

Epiberberine chloride is an alkaloid isolated from Coptis chinensis, acts as a potent AChE and BChE inhibitor, and a non-competitive BACE1 inhibitor, with IC₅₀s of 1.07, 6.03 and 8.55 μM, respectively. Epiberberine chloride has antioxidant activity, with peroxynitrite ONOO^- scavenging effect (IC₅₀, 16.83 μM), and may protect against Alzheimer disease. Epiberberine chloride inhibits the early stage of differentiation of 3T3-L1 preadipocytes, downregulates the Raf/MEK1/2/ERK1/2 and AMPKα/Akt pathways. Epiberberine has the potential effect in the research of diabetic disease.

体外研究

Epiberberine (0, 12.5, 25, or 50 μM) dose-dependently inhibits cellular triglyceride accumulation in 3T3-L1 adipocytes, with an IC₅₀ of 52.8 μM.
Epiberberine (12.5-50 μM) suppresses the Raf/MEK1/ERK1/2 and AMPKα/Akt pathways in the early stage of 3T3-L1 adipocyte differentiation.
Epiberberine (0.2, 1, 5 μg/mL) inhibits glucose uptake in HepG2 cells in a concentration-dependent manner.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究

Epiberberine (225 mg/kg, p.o. daily for 40 days) reduces body weight, food consumption, water intake, and urinary output of KK-Ay mice.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究

Epiberberine (225 mg/kg, p.o. daily for 40 days) reduces body weight, food consumption, water intake, and urinary output of KK-Ay mice.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

性状

Solid

溶解性数据

In Vitro:

DMSO : 25 mg/mL (67.24 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	2.6895 mL	13.4477 mL	26.8955 mL
5 mM	0.5379 mL	2.6895 mL	5.3791 mL
10 mM	0.2690 mL	1.3448 mL	2.6895 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in saline)
Solubility: ≥ 2.5 mg/mL (6.72 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (6.72 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明