INDY|cas:1169755-45-6|西域试剂

INDY,99.51%

产品编号：Bellancom-108476| CAS NO：1169755-45-6| 分子式：C₁₂H₁₃NO₂S| 分子量：235.30

本网站销售的所有产品仅用于工业应用或者科学研究等非医疗目的,不可用于人类或动物的临床诊断或者治疗,非药用,非食用，

货号	价格	库存与货期
Bellancom-108476	￥1500.00	杭州北京（现货）
Bellancom-108476	￥2400.00	杭州北京（现货）
Bellancom-108476	￥4800.00	杭州北京（现货）
Bellancom-108476	￥7700.00	杭州北京（现货）
Bellancom-108476	￥12300.00	杭州北京（现货）

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INDY

产品介绍

INDY 是一种有效，ATP 竞争性的 Dyrk1A 和 Dyrk1B 抑制剂，IC₅₀ 分别为 0.24 μM 和 0.23 μM。INDY 结合在酶的 ATP 口袋中，对 Dyrk1A 的 K_i 值为 0.18 μM。INDY 大大降低了原代胶质母细胞瘤 (GBM) 细胞系和神经祖细胞中正常细胞和致瘤细胞的自我更新能力。

生物活性

INDY is a potent and ATP-competitive Dyrk1A and Dyrk1B inhibitor with IC₅₀s of 0.24 μM and 0.23 μM, respectively. INDY binds in the ATP pocket of the enzyme and has a K_i value of 0.18 μM for Dyrk1A. INDY sharply reduces the self-renewal capacity of normal and tumorigenic cells in primary Glioblastoma (GBM) cell lines and neural progenitor cells.

体外研究

INDY (0.3-30 μM; 20 hours) mildly inhibits tau-phosphorylation at 3 μM, and nearly completely inhibits at 30 μM.
INDY effectively reverses the aberrant tau-phosphorylation and rescues the repressed NFAT (nuclear factor of activated T cell) signalling induced by Dyrk1A (dual-specificity tyrosine-(Y)-phosphorylation-regulated kinase 1A) overexpression.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis

Cell Line:	COS7 cells transfected with either EGFP-Dyrk1A and EGFP-tau
Concentration:	0.3, 1, 3, 10, 30 μM
Incubation Time:	20 hours
Result:	Mildly inhibited tau-phosphorylation at 3 μM, and nearly completely inhibited at 30 μM.

体内研究
(In Vivo)

ProINDY (2.5 μM) recoveres apparently normal development of the Xenopus embryo.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究

ProINDY (2.5 μM) recoveres apparently normal development of the Xenopus embryo.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究

ProINDY (2.5 μM) recoveres apparently normal development of the Xenopus embryo.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

性状

Solid

溶解性数据

In Vitro:

DMSO : ≥ 33.33 mg/mL (141.65 mM)

* "≥" means soluble, but saturation unknown.

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	4.2499 mL	21.2495 mL	42.4989 mL
5 mM	0.8500 mL	4.2499 mL	8.4998 mL
10 mM	0.4250 mL	2.1249 mL	4.2499 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： 10% DMSO 90% corn oil
Solubility: ≥ 2.5 mg/mL (10.62 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (10.62 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。