CHPG sodium salt|cas:1303993-73-8|西域试剂

CHPG sodium salt,99.17%

产品编号：Bellancom-101364A| CAS NO：1303993-73-8| 分子式：C₈H₇ClNNaO₃| 分子量：223.59

本网站销售的所有产品仅用于工业应用或者科学研究等非医疗目的,不可用于人类或动物的临床诊断或者治疗,非药用,非食用，

货号	价格	库存与货期
Bellancom-101364A	￥1100.00	杭州北京（现货）
Bellancom-101364A	￥1800.00	杭州北京（现货）
Bellancom-101364A	￥3800.00	杭州北京（现货）
Bellancom-101364A	￥6000.00	杭州北京（现货）
Bellancom-101364A	￥9600.00	杭州北京（现货）

增值税发票√顺丰快递√订货电话：18601927057

CHPG sodium salt

产品介绍

CHPG sodium salt 是一个选择性的 mGluR5 激动剂，并且通过 TSG-6/NF-κB 途径减弱 BV2 小神经胶质细胞 SO₂ 诱导的氧化应激和炎症。 CHPG sodium salt 通过 ERK 和 Akt 途径在体外和体内预防创伤性脑损伤 (TBI)。

生物活性

CHPG sodium salt is a selective mGluR5 agonist, and attenuates SO₂-induced oxidative stress and inflammation through TSG-6/NF-κB pathway in BV2 microglial cells. CHPG sodium salt protects against traumatic brain injury (TBI) in vitro and in vivo by activation of the ERK and Akt signaling pathways..

体外研究

CHPG sodium salt (10-500 µM; 24 hours) significantly increases the cell viability and decreases the LDH release after SO₂ derivatives treatment.
CHPG sodium salt (0.5 mM; 30 mins ) protects BV2 cells against SO₂-induced apoptosis.
CHPG sodium salt (0.5 mM; 30 mins) treatment alone increases the expression of TSG-6 in both mRNA and protein levels.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay

Cell Line:	BV2 microglial cells
Concentration:	10, 50, 100 and 500 µM
Incubation Time:	24 hours
Result:	Increased the cell viability.

Apoptosis Analysis

Cell Line:	BV2 microglial cells
Concentration:	0.5 mM
Incubation Time:	30 mins
Result:	Protected BV2 cells against SO₂-induced apoptosis.

Western Blot Analysis

Cell Line:	BV2 microglial cells
Concentration:	0.5 mM
Incubation Time:	30 mins
Result:	Increased the expression of TSG-6 in both mRNA and protein levels.

体内研究
(In Vivo)

CHPG sodium salt (injection; 250 nM; for 7 days) reduces significantly cerebral lesion volume.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Adult Sprague-Dawley male rats weighing 280-320 g
Dosage:	250 nM
Administration:	Injection; for 7 days
Result:	Reduced significantly cerebral lesion volume.

体内研究

CHPG sodium salt (injection; 250 nM; for 7 days) reduces significantly cerebral lesion volume.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Adult Sprague-Dawley male rats weighing 280-320 g
Dosage:	250 nM
Administration:	Injection; for 7 days
Result:	Reduced significantly cerebral lesion volume.

体内研究

CHPG sodium salt (injection; 250 nM; for 7 days) reduces significantly cerebral lesion volume.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Adult Sprague-Dawley male rats weighing 280-320 g
Dosage:	250 nM
Administration:	Injection; for 7 days
Result:	Reduced significantly cerebral lesion volume.

性状

Solid

溶解性数据

In Vitro:

DMSO : 100 mg/mL (447.25 mM; Need ultrasonic)

H₂O : 6.67 mg/mL (29.83 mM; ultrasonic and warming and heat to 80°C)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	4.4725 mL	22.3624 mL	44.7247 mL
5 mM	0.8945 mL	4.4725 mL	8.9449 mL
10 mM	0.4472 mL	2.2362 mL	4.4725 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： PBS
Solubility: 5 mg/mL (22.36 mM); Clear solution; Need ultrasonic and warming and heat to 60°C
2.

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: ≥ 2.5 mg/mL (11.18 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (11.18 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。
将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液
3.

请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in saline)
Solubility: ≥ 2.5 mg/mL (11.18 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (11.18 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
4.

请依序添加每种溶剂： 10% DMSO 90% corn oil
Solubility: ≥ 2.5 mg/mL (11.18 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (11.18 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。