K6PC-5|cas:756875-51-1|西域试剂

K6PC-5,99.85%

产品编号：Bellancom-124042| CAS NO：756875-51-1| 分子式：C₁₉H₃₇NO₄| 分子量：343.50

本网站销售的所有产品仅用于工业应用或者科学研究等非医疗目的,不可用于人类或动物的临床诊断或者治疗,非药用,非食用，

货号	价格	库存与货期
Bellancom-124042	￥1000.00	杭州北京（现货）
Bellancom-124042	￥1600.00	杭州北京（现货）
Bellancom-124042	￥3500.00	杭州北京（现货）
Bellancom-124042	￥5700.00	杭州北京（现货）
Bellancom-124042	￥9400.00	杭州北京（现货）

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K6PC-5

产品介绍

K6PC-5，一种神经酰胺衍生物，是一种鞘氨醇激酶 1 (SPHK1) 激活剂，可引起细胞内钙水平的短暂快速升高。K6PC-5 用于研究涉及异常角质形成细胞的皮肤疾病、神经退行性疾病和病毒感染。

生物活性

K6PC-5, a ceramide derivative, is a sphingosine kinase 1(SPHK1) activator and elicites a rapid transient increase in intracellular calcium levels. K6PC-5 has the potential for skin diseases involving abnormal keratinocyte, and neurodegeneration and virus infection research.

体外研究

K6PC-5 (1-10 μM; 24 h) increases the involucrin and loricrin levels in a dose-dependent manner in normal human epidermal keratinocytes (NHEKs). K6PC-5 promotes differentiation and proliferation of keratinocytes via intracellular Ca²⁺ signaling. In addition, K6PC-5 stimulates the phosphorylation of p42/44 extracellular signal-regulated kinase and c-Jun N-terminal kinase.
K6PC-5 (1-10 μM; 24 h) promotes fibroblasts proliferation and collagen synthesis in human fibroblasts. K6PC-5 induces intracellular Ca²⁺ concentration ([Ca²⁺]_i) oscillations in human fibroblasts.
K6PC-5 (10, 25, and 50 μM; 48 h) significantly attenuates EBOV-induced infection in EBOV-infected EA.hy926 cells. K6PC-5 significantly reduces the virus titers in supernatants of infected cells and strikingly decreased the amount of VP40 in a concentration-dependent manner.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis

Cell Line:	Normal human epidermal keratinocytes (NHEKs)
Concentration:	1 μM, 5 μM, 10 μM
Incubation Time:	24 h
Result:	Increased the involucrin and loricrin levels in a dose-dependent manner.

Cell Proliferation Assay

Cell Line:	Human fibroblasts
Concentration:	1 μM, 5 μM, 10 μM
Incubation Time:	24 h
Result:	Promoted fibroblast proliferation and procollagen production in human fibroblasts significantly.

体内研究
(In Vivo)

In intrinsically aged hairless mice (56 weeks old), 1% K6PC-5 is applied topically for 2 weeks. This K6PC-5 treatment significantly increases both the number of dermal fibroblasts and collagen production. As a consequence, dermal thickness also increased significantly.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Intrinsically aged hairless mice (56 weeks old)
Dosage:	1% (vehicle (PEG:EtOH = 7:3))
Administration:	Topical application; twice daily for 2 weeks
Result:	Enhanced fibroblast proliferation, collagen production, and eventually increased dermal thickness.

体内研究

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Intrinsically aged hairless mice (56 weeks old)
Dosage:	1% (vehicle (PEG:EtOH = 7:3))
Administration:	Topical application; twice daily for 2 weeks
Result:	Enhanced fibroblast proliferation, collagen production, and eventually increased dermal thickness.

体内研究

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Intrinsically aged hairless mice (56 weeks old)
Dosage:	1% (vehicle (PEG:EtOH = 7:3))
Administration:	Topical application; twice daily for 2 weeks
Result:	Enhanced fibroblast proliferation, collagen production, and eventually increased dermal thickness.

性状

Solid

溶解性数据

In Vitro:

DMSO : 100 mg/mL (291.12 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	2.9112 mL	14.5560 mL	29.1121 mL
5 mM	0.5822 mL	2.9112 mL	5.8224 mL
10 mM	0.2911 mL	1.4556 mL	2.9112 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: ≥ 2.5 mg/mL (7.28 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (7.28 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。
将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液
2.

请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in saline)
Solubility: ≥ 2.5 mg/mL (7.28 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (7.28 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
3.

请依序添加每种溶剂： 10% DMSO 90% corn oil
Solubility: ≥ 2.5 mg/mL (7.28 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (7.28 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。