7BIO 7-Bromoindirubin-3-Oxime|cas:916440-85-2|西域试剂

7BIO 7-Bromoindirubin-3-Oxime,98.0%

产品编号：Bellancom-121035| CAS NO：916440-85-2| 分子式：C₁₆H₁₀BrN₃O₂| 分子量：356.17

本网站销售的所有产品仅用于工业应用或者科学研究等非医疗目的,不可用于人类或动物的临床诊断或者治疗,非药用,非食用，

货号	价格	库存与货期
Bellancom-121035	￥800.00	杭州北京（现货）
Bellancom-121035	￥1200.00	杭州北京（现货）
Bellancom-121035	￥2400.00	杭州北京（现货）
Bellancom-121035	￥3800.00	杭州北京（现货）
Bellancom-121035	￥6000.00	杭州北京（现货）

增值税发票√顺丰快递√订货电话：18601927057

7BIO 7-Bromoindirubin-3-Oxime

产品介绍

7BIO (7-Bromoindirubin-3-Oxime) 是靛玉红的衍生物。7BIO (7-Bromoindirubin-3-Oxime) 对细胞周期蛋白依赖性激酶5 (CDK5) 和糖原合酶激酶-3β (GSK3β) 具有抑制作用。7BIO (7-Bromoindirubin-3-Oxime) 抑制 Aβ 寡聚体诱导的神经炎症、突触损伤、tau 过度磷酸化、星形胶质细胞和小胶质细胞的活化，并减轻 Aβ 寡聚体诱导的小鼠认知障碍。

生物活性

7BIO (7-Bromoindirubin-3-Oxime) is the derivate of indirubin. 7BIO (7-Bromoindirubin-3-Oxime) has inhibitory effects against cyclin-dependent kinase-5 (CDK5) and glycogen synthase kinase-3β (GSK3β). 7BIO (7-Bromoindirubin-3-Oxime) inhibits Aβ oligomer-induced neuroinflammation, synaptic impairments, tau hyper-phosphorylation, activation of astrocytes and microglia, and attenuates Aβ oligomer-induced cognitive impairments in mice.

体外研究

7BIO (1 and 10 μM; 24 hours) has neuroprotective effects and can prevent, but cannot rescue Aβ1−42 oligomer-induced cell death in SH-SY5Y cells.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay

Cell Line:	SH-SY5Y cells
Concentration:	1 and 10 μM
Incubation Time:	24 hours
Result:	Inhibit Aβ oligomer-induced neuronal death. 7BIO prevented, but did not rescue Aβ1−42 oligomer-induced cell death in SH-SY5Y cells.

体内研究
(In Vivo)

7Bio (2.3, 7.0, and 23.3 μg/kg; bilateral ventricle injection) significantly attenuates Aβ oligomer-induced impairment of recognition, spatial learning and memory in mice.
7Bio (2.3, 7.0, and 23.3 μg/kg; bilateral ventricle injection) decreases Aβ oligomer-induced increase of TNF-α and IL-6 production in the brain and the expression of synapsin-1 and PSD-95 in the hippocampal region of mice.
7Bio (2.3, 7.0, and 23.3 μg/kg; bilateral ventricle injection) attenuates Aβ oligomer-induced increase expression of pTau, activation of microglia and astrogliosis in the brain of mice.
7Bio (2.3, 7.0, and 23.3 μg/kg; bilateral ventricle injection) prevents decreased expression of pSer9-GSK3β and has no significant effects on the Tau protein level.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	8 weeks mice (30 g)
Dosage:	2.3, 7.0, and 23.3 μg/kg
Administration:	bilateral ventricle injection
Result:	Attenuates Aβ oligomer-induced impairment of recognition, spatial learning and memory in mice.

体内研究

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	8 weeks mice (30 g)
Dosage:	2.3, 7.0, and 23.3 μg/kg
Administration:	bilateral ventricle injection
Result:	Attenuates Aβ oligomer-induced impairment of recognition, spatial learning and memory in mice.

体内研究

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	8 weeks mice (30 g)
Dosage:	2.3, 7.0, and 23.3 μg/kg
Administration:	bilateral ventricle injection
Result:	Attenuates Aβ oligomer-induced impairment of recognition, spatial learning and memory in mice.

性状

Solid

溶解性数据

In Vitro:

DMSO : 250 mg/mL (701.91 mM; Need ultrasonic)

Ethanol : 50 mg/mL (140.38 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	2.8076 mL	14.0382 mL	28.0765 mL
5 mM	0.5615 mL	2.8076 mL	5.6153 mL
10 mM	0.2808 mL	1.4038 mL	2.8076 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： 10% EtOH 40% PEG300 5% Tween-80 45% saline
Solubility: ≥ 2.5 mg/mL (7.02 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (7.02 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 EtOH 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。
将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液
2.

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: ≥ 2.08 mg/mL (5.84 mM); Clear solution

此方案可获得 ≥ 2.08 mg/mL (5.84 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。
将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液