Real Thiol|cas:2280796-90-7|西域试剂

Real Thiol,99.87%

产品编号：Bellancom-108715| CAS NO：2280796-90-7| 分子式：C₂₀H₁₇N₃O₇| 分子量：411.36

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货号	包装	价格	库存与货期	购买量	操作
Bellancom-108715		￥3500.00	杭州北京（现货）

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Real Thiol

产品介绍

Real Thiol 是一种基于可逆反应的荧光探针，可以定量监测活细胞中谷胱甘肽的实时动态变化。储存方式：避光。

生物活性

Real Thiol is a reversible reaction-based fluorescent probe which can quantitatively monitor the real-time glutathione dynamics in living cells. Storage: protect from light.

体外研究

Real Thiol (RT) shows ratiometric fluorescence responses with a wide dynamic range when reacting with Glutathione (GSH). Real Thiol and its GSH adduct (RT-GSH) shows fluorescence maxima at 487 and 562 nm with excitation wavelengths at 405 and 488 nm, respectively. The K_d for the reaction between Real Thiol and GSH is 3.7 mM. Real Thiol has much improved quantum yields and photostability. The wide dynamic range of Real Thiol allows for monitoring of the GSH level changes within 1 to 10 mM in both directions in living cells. Gel permeation chromatography (GPC) analysis showes that 10% of Real Thiol reacts with thiolated proteins while 90% of Real Thiol reacts with GSH.
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Real Thiol (RT) is dissolved in DMSO to obtain a 10 mM stock solutions and further diluted before use.
2. Cells are cultured in 10% FBS DMEM containing different concentrations of Buthionine sulfoximine (0.9, 1.9, 3.9, 7.8, 15.6, 31.3, 62.5, 125, 250 and 500 μM) for 72 h and harvested for analysis.
3. Cells are then suspended in fresh medium containing 1 μM of Real Thiol probe as single-cell suspensions, and keep the staining time between 9 and 10 min for each sample.
4. Analyze sample on a flow cytometer. PE and pacific-blue channels were selected to measure the fluorescence intensities of unreacted and reacted Real Thiol, respectively.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究

性状

Solid

溶解性数据

In Vitro:

DMSO : ≥ 101 mg/mL (245.53 mM)

* "≥" means soluble, but saturation unknown.

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	2.4310 mL	12.1548 mL	24.3096 mL
5 mM	0.4862 mL	2.4310 mL	4.8619 mL
10 mM	0.2431 mL	1.2155 mL	2.4310 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式