4'-Chlorodiazepam (Ro 5-4864), a benzodiazepine derivative of Diazepam, is a potent mitochondrial translocator protein 18kDa (TSPO) ligand. 4'-Chlorodiazepam shows neuroprotective effects.

4'-Chlorodiazepam is neuroprotective against amyloid-beta (Aβ) in SHSY-5Y neuroblastoma cells. Aβ decreases the cell viability of organotypic hippocampal cultures, while 4'-Chlorodiazepam has a neuroprotective effect when administered at 100nM and 1000nM. The neuroprotective effects of 4'-Chlorodiazepam against Aβ are associated with an increased expression of superoxide dismutase (SOD) .
4'-Chlorodiazepam preserves cell viability and reduces nuclear fragmentation in glucose-deprived cells. These effects are accompanied by a reduced production of free radicals and maintenance of mitochondrial functions in cells treated with 4'-Chlorodiazepam.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

• . B D Arbo, et al. 4'-Chlorodiazepam is neuroprotective against amyloid-beta in organotypic hippocampal cultures. J Steroid Biochem Mol Biol. 2017 Jul;171:281-287.  [Content Brief]

  . Eliana Baez, et al. 4'-Chlorodiazepam Protects Mitochondria in T98G Astrocyte Cell Line from Glucose Deprivation. Neurotox Res. 2017 Aug;32(2):163-171.  [Content Brief]