HBC514|cas:2530161-67-0|西域试剂

HBC514,98.0%

产品编号：Bellancom-133523| CAS NO：2530161-67-0| 分子式：C₂₁H₂₂N₄| 分子量：330.43

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货号	价格	库存与货期
Bellancom-133523	￥3500.00	杭州北京（现货）
Bellancom-133523	￥6350.00	杭州北京（现货）
Bellancom-133523	￥11500.00	杭州北京（现货）
Bellancom-133523	￥18500.00	杭州北京（现货）

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HBC514

产品介绍

HBC514 本身是一种非荧光的 HBC 模拟物，但在与 Pepper RNA 适配体形成紧密复合物后可以发出强烈的绿色荧光，用于 RNA 标记。在活的 Pepper 标记的细胞中，HBC514-Pepper complex 可以使 RNAs 可视化，并且可以通过简单的洗涤和染色灵活地改变荧光。储存方式：避光。

生物活性

HBC514 is a nonfluorescent HBC-analog but emits strong green fluorescence upon forming a tight complex with Pepper RNA aptamer. HBC514-Pepper complex enables visualization of RNAs and the fluorescences can be altered flexibly by simple washing and staining in living Pepper-tagged cells. Storage: protect from light.

体外研究

HBC is a GFP fluorophore-like synthetic dye with a structurally rigid electron acceptor and a strong electron donor, nonfluorescent in solution. ‘Pepper’ is able to enhance HBC’s fluorescence more than 3,000-fold and exhibits bright fluorescence when expressed in living cells.
‘Pepper’ has reduced Mg²⁺ sensitivity after multiple rounds of optimization, displays a well-defined fluorescent profile with an excitation maximum at 485nm, an emission maximum at 530nm, and excellent brightness.
HBC514 (2-10 μM) is altered flexibly by simple washing and staining by Pepper based CRISPR imaging in live HeLa cells (expressing sgRNA-Pep1 and dCas9).

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究

性状

Solid

溶解性数据

In Vitro:

DMSO : 25 mg/mL (75.66 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	3.0264 mL	15.1318 mL	30.2636 mL
5 mM	0.6053 mL	3.0264 mL	6.0527 mL
10 mM	0.3026 mL	1.5132 mL	3.0264 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式