BD750|cas:892686-59-8|西域试剂

BD750,99.79%

产品编号：Bellancom-131140| CAS NO：892686-59-8| 分子式：C₁₄H₁₃N₃OS| 分子量：271.34

本网站销售的所有产品仅用于工业应用或者科学研究等非医疗目的,不可用于人类或动物的临床诊断或者治疗,非药用,非食用，

货号	价格	库存与货期
Bellancom-131140	￥600.00	杭州北京（现货）
Bellancom-131140	￥1000.00	杭州北京（现货）
Bellancom-131140	￥1800.00	杭州北京（现货）
Bellancom-131140	￥3000.00	杭州北京（现货）
Bellancom-131140	￥5000.00	杭州北京（现货）

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BD750

产品介绍

BD750 是一个有效的免疫抑制剂，是JAK3/STAT5 的抑制剂，可抑制 IL-2 诱导的、JAK3/STAT5 依赖的 T 细胞增殖，其抑制小鼠和人 T 细胞增殖的IC₅₀ 值分别为 1.5 μM 和 1.1 μM。

生物活性

BD750, an effective immunosuppressant and a JAK3/STAT5 inhibitor, inhibits IL-2-induced JAK3/STAT5-dependent T cell proliferation, with IC₅₀ values of 1.5 μM and 1.1 μM in mouse and human T cells, respectively.

体外研究

BD750 inhibits human T cell proliferation stimulated either by anti-CD3/anti-CD28 mAbs or by alloantigen in a dose-dependent manner with IC₅₀ values of 1.1 ± 0.2 μM and 1.3 ± 0.2 μM respectively.
BD750 also inhibits ConA, PMA/ionomycine or alloantigen-induced mouse T cell proliferation and PHA or PMA/ionomycine-induced human T cell proliferation.
BD750 (5 or 20 μM) inhibits the LPS-induced JAK-STAT5 signaling in DC.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis

Cell Line:	Purified immature DCs.
Concentration:	1, 5 or 20 μM.
Incubation Time:	12 h.
Result:	Had fewer small dendrites and smaller clusters than typical mDCs (5 or 20 μM). At a higher dose significantly reduced the levels of LPS-stimulated IL-6, IL-12, TNF-α, IL-1β and IL-23 production by DCs.

体内研究
(In Vivo)

BD750 can induce tolerogenic dendritic cells (tolDC) and their function in experimental autoimmune encephalitis (EAE) in mice.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Female C57BL/6 mice (10 wks old, 19-21 g).
Dosage:	20 μM.
Administration:	IV, treated DC on d 7, 11 and 15 post the first PTX injection (dpi 7, 11 and 15).
Result:	Significantly reduced the frequency of Th1 and Th17 cells and increased the percentage of Tregs compared with mice receiving PBS.

体内研究

BD750 can induce tolerogenic dendritic cells (tolDC) and their function in experimental autoimmune encephalitis (EAE) in mice.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Female C57BL/6 mice (10 wks old, 19-21 g).
Dosage:	20 μM.
Administration:	IV, treated DC on d 7, 11 and 15 post the first PTX injection (dpi 7, 11 and 15).
Result:	Significantly reduced the frequency of Th1 and Th17 cells and increased the percentage of Tregs compared with mice receiving PBS.

体内研究

BD750 can induce tolerogenic dendritic cells (tolDC) and their function in experimental autoimmune encephalitis (EAE) in mice.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Female C57BL/6 mice (10 wks old, 19-21 g).
Dosage:	20 μM.
Administration:	IV, treated DC on d 7, 11 and 15 post the first PTX injection (dpi 7, 11 and 15).
Result:	Significantly reduced the frequency of Th1 and Th17 cells and increased the percentage of Tregs compared with mice receiving PBS.

性状

Solid

溶解性数据

In Vitro:

DMSO : 125 mg/mL (460.68 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	3.6854 mL	18.4271 mL	36.8541 mL
5 mM	0.7371 mL	3.6854 mL	7.3708 mL
10 mM	0.3685 mL	1.8427 mL	3.6854 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in saline)
Solubility: ≥ 2.08 mg/mL (7.67 mM); Clear solution

此方案可获得 ≥ 2.08 mg/mL (7.67 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明