KMG-104 is a highly selective fluorescent Mg²⁺ probe. KMG-104 has been used widely and revealed Mg²⁺ mobilization in cytoplasm in various types of cells.

KMG-104 is useful for confocal laser scanning microscopy because it is excited at 490 nm, and emitted fluorescence around 510 nm. Fluorescent intensity of KMG-104 increases with [Mg] increase, and it shows no response to Na, K, and Ca; the dissociation constant (K_d) for Mg is 3 mM. KMG-104 is able to trace only [Mg] change inphysiological conditions.
PC12 cells are loaded with a novel Mg indicator KMG-104 and Ca indicator fura-2, and intracellular Mg is studied in the endoplasmic reticulums (ERs), mitochondria, and Mg-ATP.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

• . Kubota T, et al. Investigation of intracellular magnesium mobilization pathways I PC12 cells B simultaneous Mg-Ca fluorescent imaging. J Am Coll Nutr. 2004;23(6):742S-4S.  [Content Brief]

  . Fujii T, et al. Design and synthesis of a FlAsH-type Mg2+ fluorescent probe for specific protein labeling. J Am Chem Soc. 2014;136(6):2374-2381.  [Content Brief]