PBT 1033 PBT 2|cas:747408-78-2|西域试剂

PBT 1033 PBT 2,98.01%

产品编号：Bellancom-105321| CAS NO：747408-78-2| 分子式：C₁₂H₁₂Cl₂N₂O| 分子量：271.14

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货号	价格	库存与货期
Bellancom-105321	￥1400.00	杭州北京（现货）
Bellancom-105321	￥2200.00	杭州北京（现货）
Bellancom-105321	￥4500.00	杭州北京（现货）
Bellancom-105321	￥7200.00	杭州北京（现货）

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PBT 1033 PBT 2

产品介绍

PBT 1033 (PBT 2) 是一种具有口服活性的铜/锌离子载体。PBT 1033 可恢复阿尔茨海默病 (AD) 小鼠模型中小鼠的认知能力。PB 1033 还对革兰氏阳性菌具有抗菌活性。

生物活性

PBT 1033 (PBT 2) is an orally active copper/zinc ionophore. PBT 1033 restores cognition in mouse models of Alzheimer's disease (AD). PB 1033 also has antibacterial activity against Gram-positive bacteria.

体外研究

PB 1033 displays antibacterial activity against S. uberis, with a MIC value of 14.5 μM.
PBT2 (1, 3 and 7.5 μM, 6 h) protects neurons against glutamate-induced excitotoxicity.
PBT2 (10 μM, 1 or 6 h) reduces NMDAR-mediated Ca2+ flux in mouse cortical neurons.
PBT2 (0-10 μM, 1 h) increases GSK3α/β phosphorylation in SH-SY5Y cells^[4].
PBT2 (20 μM, 1 h) prevents the formation of Zn-induced protease resistant Aβ aggregates^[5].

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis^[4]

Cell Line:	SH-SY5Y cells
Concentration:	0-10 μM
Incubation Time:	1 h
Result:	Increased in cellular levels of GSK3α/β phosphorylated at the inhibitory serine 21/9 residue (ser21/9 on GSK3α/β).

体内研究
(In Vivo)

PBT 1033 (30 mg/kg/d, p.o., 11 days) restores biochemical substrates of learning/memory in a mouse model of alzheimer's disease^[5].

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Female Tg2576 mice^[5]
Dosage:	30 mg/kg/d
Administration:	Oral administration, 11 days
Result:	Increased hippocampal apical spine density, basal spine density.

体内研究

PBT 1033 (30 mg/kg/d, p.o., 11 days) restores biochemical substrates of learning/memory in a mouse model of alzheimer's disease^[5].

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Female Tg2576 mice^[5]
Dosage:	30 mg/kg/d
Administration:	Oral administration, 11 days
Result:	Increased hippocampal apical spine density, basal spine density.

体内研究

PBT 1033 (30 mg/kg/d, p.o., 11 days) restores biochemical substrates of learning/memory in a mouse model of alzheimer's disease^[5].

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Female Tg2576 mice^[5]
Dosage:	30 mg/kg/d
Administration:	Oral administration, 11 days
Result:	Increased hippocampal apical spine density, basal spine density.

性状

Solid

溶解性数据

In Vitro:

DMSO : 100 mg/mL (368.81 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	3.6881 mL	18.4407 mL	36.8813 mL
5 mM	0.7376 mL	3.6881 mL	7.3763 mL
10 mM	0.3688 mL	1.8441 mL	3.6881 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in saline)
Solubility: ≥ 2.5 mg/mL (9.22 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (9.22 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明