hnRNPK-IN-1|cas:2313528-04-8|西域试剂

hnRNPK-IN-1,97.11%

产品编号：Bellancom-135691| CAS NO：2313528-04-8| 分子式：C₂₃H₂₁N₃O₅| 分子量：419.43

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货号	价格	库存与货期
Bellancom-135691	￥5500.00	杭州北京（现货）
Bellancom-135691	￥8800.00	杭州北京（现货）
Bellancom-135691	￥18000.00	杭州北京（现货）
Bellancom-135691	￥30000.00	杭州北京（现货）
Bellancom-135691	￥46000.00	杭州北京（现货）

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hnRNPK-IN-1

产品介绍

hnRNPK-IN-1 是一种异质核糖核蛋白 K (hnRNPK) 结合配体，用 SPR 和 MST 测量的 K_d 值分别为 4.6 μM 和 2.6 μM。hnRNPK-IN-1 通过破坏 hnRNPK 和 c-myc 启动子的结合来抑制 c-myc 转录。hnRNPK-IN-1诱导 Hela 细胞凋亡 (apoptosis)，并具有很强的抗肿瘤活性。

生物活性

hnRNPK-IN-1 is a heterogeneous nuclear ribonucleoprotein K (hnRNPK) binding ligand with K_d values of 4.6 μM and 2.6 μM measured with SPR and MST, respectively. hnRNPK-IN-1 inhibits c-myc transcription by disrupting the binding of hnRNPK and c-myc promoter. hnRNPK-IN-1 induces Hela cells apoptosis and has strongly anti-tumor activities.

体外研究

hnRNPK-IN-1 (Compound 25; 1.25-5 μM; 24 hours) treatment induces Hela cells apoptosis could be due to its repression of cmyc transcription.
hnRNPK-IN-1 (1.25-5 μM; 48 hours) treatment down-regulates c-myc gene transcription and expression in Hela cells in a dose-dependent manner.
hnRNPK-IN-1 shows a selective anti-proliferative effect on human cancer cell lines (Siha, A549, Hela, U2OS, A375, HuH7 and HEK293 cells) with IC50 values ranged from 1.36 to 3.59 μM.
hnRNPK-IN-1 could selectively bind to hnRNP K without significant interaction with c-myc promoter DNA. The binding of hnRNPK-IN-1 to hnRNP K could disrupt the interaction between hnRNP K and c-myc promoter DNA in vitro and in cells.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Apoptosis Analysis

Cell Line:	Hela cells
Concentration:	1.25 μM, 2.5 μM, 5 μM
Incubation Time:	24 hours
Result:	Induced Hela cells apoptosis and apoptosis-related protein expressions.

RT-PCR

Cell Line:	Hela cells
Concentration:	1.25 μM, 2.5 μM, 5 μM
Incubation Time:	48 hours
Result:	Caused a reduction of c-myc mRNA in a dose-dependent manner in Hela cells.

Western Blot Analysis

Cell Line:	Hela cells
Concentration:	1.25 μM, 2.5 μM, 5 μM
Incubation Time:	48 hours
Result:	The expression levels of c-MYC also decreased in a dose-dependent manner.

体内研究
(In Vivo)

hnRNPK-IN-1 (Compound 25; 6.7-20 mg/kg; i.p.; once a day; for three weeks) exhibits good tumor growth inhibition in a Hela xenograft tumor model.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	BALB/c female nude mice (five weeks old) bearing Hela cells
Dosage:	6.7 mg/kg, 20 mg/kg
Administration:	Intraperitoneal injection; once a day; for three weeks
Result:	Inhibited tumor growth in a Hela cervical xenograft.

体内研究

hnRNPK-IN-1 (Compound 25; 6.7-20 mg/kg; i.p.; once a day; for three weeks) exhibits good tumor growth inhibition in a Hela xenograft tumor model.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	BALB/c female nude mice (five weeks old) bearing Hela cells
Dosage:	6.7 mg/kg, 20 mg/kg
Administration:	Intraperitoneal injection; once a day; for three weeks
Result:	Inhibited tumor growth in a Hela cervical xenograft.

体内研究

hnRNPK-IN-1 (Compound 25; 6.7-20 mg/kg; i.p.; once a day; for three weeks) exhibits good tumor growth inhibition in a Hela xenograft tumor model.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	BALB/c female nude mice (five weeks old) bearing Hela cells
Dosage:	6.7 mg/kg, 20 mg/kg
Administration:	Intraperitoneal injection; once a day; for three weeks
Result:	Inhibited tumor growth in a Hela cervical xenograft.

性状

Solid

溶解性数据

In Vitro:

DMSO : 5 mg/mL (11.92 mM; ultrasonic and warming and heat to 80°C)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	2.3842 mL	11.9209 mL	23.8419 mL
5 mM	0.4768 mL	2.3842 mL	4.7684 mL
10 mM	0.2384 mL	1.1921 mL	2.3842 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式