CREBtide|cas:149155-45-3|西域试剂

CREBtide,98.02%

产品编号：Bellancom-P1595| CAS NO：149155-45-3| 分子式：C₇₃H₁₂₉N₂₉O₁₉| 分子量：1716.99

本网站销售的所有产品仅用于工业应用或者科学研究等非医疗目的,不可用于人类或动物的临床诊断或者治疗,非药用,非食用，

货号	价格	库存与货期
Bellancom-P1595	￥800.00	杭州北京（现货）
Bellancom-P1595	￥2800.00	杭州北京（现货）
Bellancom-P1595	￥4400.00	杭州北京（现货）

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CREBtide

产品介绍

CREBtide 是一种合成的含 13 个氨基酸的肽，作为一种 PKA 底物。

生物活性

CREBtide, a synthetic 13 amino acid peptide, has been reported as a PKA substrate.

体外研究

delta-CREB is a spliced variant of cAMP response element binding protein (CREB). CREBtide (KRREILSRRPSYR), a synthetic peptide based on the phosphorylation sequence in delta-CREB. delta-CREB and CREBtide are tested as substrates of cAMP-dependent protein kinase (cAK). The apparent K_m of CREBtide phosphorylation by cAK is 3.9 μM, which is 10-fold lower than that of Kemptide (K_m=39 μM), the synthetic peptide substrate most often employed for cAK measurement. The V_max values are 12.4 mumol/(min.mg) for CREBtide and 9.8 mumol/(min.mg) for Kemptide. The apparent K_m of CREBtide phosphorylation by cGMP-dependent protein kinase (cGK) is 2.9 μM and the V_max value is 3.2 mumol/(min.mg). Both delta-CREB and CREBtide are phosphorylated at a much slower rate by cGK as compared with cAK, implying that the high cAK/cGK specificity exhibits by delta-CREB is retained by the peptide.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究

性状

Solid

溶解性数据

In Vitro:

H₂O : 100 mg/mL (58.24 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	0.5824 mL	2.9121 mL	5.8241 mL
5 mM	0.1165 mL	0.5824 mL	1.1648 mL
10 mM	0.0582 mL	0.2912 mL	0.5824 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶