LR-90|cas:245075-84-7|西域试剂

LR-90,99.59%

产品编号：Bellancom-76383| CAS NO：245075-84-7| 分子式：C₃₅H₃₄Cl₂N₄O₈| 分子量：709.57

本网站销售的所有产品仅用于工业应用或者科学研究等非医疗目的,不可用于人类或动物的临床诊断或者治疗,非药用,非食用，

货号	价格	库存与货期
Bellancom-76383	￥2000.00	杭州北京（现货）
Bellancom-76383	￥3500.00	杭州北京（现货）
Bellancom-76383	￥10500.00	杭州北京（现货）
Bellancom-76383	￥16800.00	杭州北京（现货）

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LR-90

产品介绍

LR-90 是晚期糖基化终产物 (advanced glycation end product (AGE)) 抑制剂，能够抑制人单核细胞的炎症反应。LR-90 也可用于糖尿病动物模型研究。

生物活性

LR-90 is an advanced glycation end product (AGE) inhibitor, inhibits inflammatory responses in human monocytes. LR-90 is also used in the research of diabetic animal model.

体外研究

LR-90 (0, 25, 50, 100, and 200 μM) inhibits RAGE, MCP-1, COX-2, IP-10 and NOX2 mRNA expression in THP-1 cells in a dose-dependent manner, after pretreatment 1 h befor S100b stimulatation for 4 hours.
LR-90 (0, 25, 50, 100, and 200 μM) dose-dependently and significantly blocks THP-1 cells adherence to endothelial cells after pretreatment 1 h befor S100b stimulatation for 24 hours.
LR-90 (0, 25, 50, 100, and 200 μM, for 24 hours) shows no effect on the cell viability of THP-1 cells.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay

Cell Line:	THP-1 cells
Concentration:	0, 25, 50, 100, and 200 μM
Incubation Time:	24 hours
Result:	Showed no cytotoxicity to THP-1 cells.

RT-PCR

Cell Line:	THP-1 cells
Concentration:	0, 25, 50, 100, and 200 μM
Incubation Time:	One hour before S100b addition for 4 hours
Result:	Dose-dependently inhibited mRNA expression of RAGE, MCP-1, COX-2, IP-10, and NOX2 stimulated with S100b.

体内研究
(In Vivo)

LR-90 (50 mg/L, p.o. for 27 weeks) significantly reduces plasma lipids, modestly affects hyperglycaemia in ZDF rats.
LR-90 (50 mg/L) decreases renal AGE, AGER and lipid peroxidation.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male ZDF rats (13 to 40 weeks)
Dosage:	50 mg/L
Administration:	P.O. for 27 weeks
Result:	Significantly reduced plasma triacylglycerol and cholesterol by ∼55% and ∼30%, respectively. Modestly affected hyperglycaemia and blood pressure. Lowered the body weight.

体内研究

LR-90 (50 mg/L, p.o. for 27 weeks) significantly reduces plasma lipids, modestly affects hyperglycaemia in ZDF rats.
LR-90 (50 mg/L) decreases renal AGE, AGER and lipid peroxidation.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male ZDF rats (13 to 40 weeks)
Dosage:	50 mg/L
Administration:	P.O. for 27 weeks
Result:	Significantly reduced plasma triacylglycerol and cholesterol by ∼55% and ∼30%, respectively. Modestly affected hyperglycaemia and blood pressure. Lowered the body weight.

体内研究

LR-90 (50 mg/L, p.o. for 27 weeks) significantly reduces plasma lipids, modestly affects hyperglycaemia in ZDF rats.
LR-90 (50 mg/L) decreases renal AGE, AGER and lipid peroxidation.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male ZDF rats (13 to 40 weeks)
Dosage:	50 mg/L
Administration:	P.O. for 27 weeks
Result:	Significantly reduced plasma triacylglycerol and cholesterol by ∼55% and ∼30%, respectively. Modestly affected hyperglycaemia and blood pressure. Lowered the body weight.

性状

Solid

溶解性数据

In Vitro:

DMSO : ≥ 100 mg/mL (140.93 mM)

* "≥" means soluble, but saturation unknown.

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	1.4093 mL	7.0465 mL	14.0930 mL
5 mM	0.2819 mL	1.4093 mL	2.8186 mL
10 mM	0.1409 mL	0.7047 mL	1.4093 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: ≥ 2.5 mg/mL (3.52 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (3.52 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。
将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液
2.

请依序添加每种溶剂： 10% DMSO 90% corn oil
Solubility: ≥ 2.5 mg/mL (3.52 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (3.52 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。