G15|cas:1161002-05-6|西域试剂

G15,99.0%

产品编号：Bellancom-103449| CAS NO：1161002-05-6| 分子式：C₁₉H₁₆BrNO₂| 分子量：370.24

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货号	价格	库存与货期
Bellancom-103449	￥712.00	杭州北京（现货）
Bellancom-103449	￥1235.00	杭州北京（现货）
Bellancom-103449	￥3800.00	杭州北京（现货）
Bellancom-103449	￥6080.00	杭州北京（现货）

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G15

产品介绍

G15 是一种高亲和力和选择性 G 蛋白偶联雌激素受体 (GPER/GPR30) 拮抗剂，K_i 值为 20 nM。

生物活性

G15 is a high affinity and selective G-protein-coupled estrogen receptor (GPER/GPR30) antagonist with a K_i of 20 nM.

体外研究

G15 (0.1-10 μM; 2 days) inhibits GPER-mediated proliferation stimulated by 17β-estradiol (E2) in A549 and H1793 cell lines.
G15 (1 μM; 48 hours) inhibits the response of GPER stimulated by E2 and G1 in A549 and H1793 cell lines.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay

Cell Line:	A549, H1793 cell lines
Concentration:	0.1, 1, 10 μM (combination with 10 nM E2)
Incubation Time:	2 days
Result:	Inhibited GPER-mediated proliferation stimulated by E2.

Western Blot Analysis

Cell Line:	A549, H1793 cell lines
Concentration:	1 μM (combination with 10 nM E2 and 10 nM G1)
Incubation Time:	48 hours
Result:	Inhibited the response of GPER stimulated by E2 and G1.

体内研究
(In Vivo)

G15 (1.46 mg/kg; i.h.; twice a week for 14 weeks) decreases the number of tumor nodules and tumor index increased by the E2 or G1 group in urethane-induced adenocarcinoma mice.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Four-week-old female Kunming mice (Urethane-induced adenocarcinoma)
Dosage:	1.46 mg/kg (combination with E2, 0.09 mg/kg and fulvestrant (Ful), 2.4 mg/kg)
Administration:	Subcutaneous injection; twice a week for 14 weeks
Result:	The number of tumor nodules decreased in the E2+Ful+G15 group.

体内研究

G15 (1.46 mg/kg; i.h.; twice a week for 14 weeks) decreases the number of tumor nodules and tumor index increased by the E2 or G1 group in urethane-induced adenocarcinoma mice.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Four-week-old female Kunming mice (Urethane-induced adenocarcinoma)
Dosage:	1.46 mg/kg (combination with E2, 0.09 mg/kg and fulvestrant (Ful), 2.4 mg/kg)
Administration:	Subcutaneous injection; twice a week for 14 weeks
Result:	The number of tumor nodules decreased in the E2+Ful+G15 group.

体内研究

G15 (1.46 mg/kg; i.h.; twice a week for 14 weeks) decreases the number of tumor nodules and tumor index increased by the E2 or G1 group in urethane-induced adenocarcinoma mice.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Four-week-old female Kunming mice (Urethane-induced adenocarcinoma)
Dosage:	1.46 mg/kg (combination with E2, 0.09 mg/kg and fulvestrant (Ful), 2.4 mg/kg)
Administration:	Subcutaneous injection; twice a week for 14 weeks
Result:	The number of tumor nodules decreased in the E2+Ful+G15 group.

性状

Solid

溶解性数据

In Vitro:

DMSO : 41.67 mg/mL (112.55 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	2.7010 mL	13.5048 mL	27.0095 mL
5 mM	0.5402 mL	2.7010 mL	5.4019 mL
10 mM	0.2701 mL	1.3505 mL	2.7010 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: ≥ 2.08 mg/mL (5.62 mM); Clear solution

此方案可获得 ≥ 2.08 mg/mL (5.62 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。
将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液
2.

请依序添加每种溶剂： 10% DMSO 90% corn oil
Solubility: ≥ 2.08 mg/mL (5.62 mM); Clear solution

此方案可获得 ≥ 2.08 mg/mL (5.62 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。