FLAG peptide|cas:98849-88-8|西域试剂

FLAG peptide,99.51%

产品编号：Bellancom-P0223| CAS NO：98849-88-8| 分子式：C₄₁H₆₀N₁₀O₂₀| 分子量：1012.97

FLAG peptide是一个多肽（Asp-Ty-rLys-Asp-Asp-Asp-Asp-Lys）由八个氨基酸组成，具有肠激酶切割位点，可用于抗体介导的重组蛋白识别和纯化。FLAG peptide是一种高酸性八肽，可以在N端融合到目标蛋白质上。作为一种非常亲水的肽，Flag-tag具有很高的表面可能性。在Ca2+存在下，可通过免疫亲和柱捕获标志融合蛋白，并在低浓度，中性pH和接近生理条件下用EDTA洗脱。

本网站销售的所有产品仅用于工业应用或者科学研究等非医疗目的,不可用于人类或动物的临床诊断或者治疗,非药用,非食用，

货号	价格	库存与货期
Bellancom-P0223	￥500.00	杭州北京（现货）
Bellancom-P0223	￥900.00	杭州北京（现货）
Bellancom-P0223	￥1300.00	杭州北京（现货）
Bellancom-P0223	￥2390.00	杭州北京（现货）

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FLAG peptide

产品介绍

FLAG peptide 是一种八个氨基酸组成的多肽 (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys)，具有肠激酶切割位点。FLAG peptide 用于抗体介导的重组蛋白的识别和纯化。

生物活性

FLAG peptide is an eight amino acids peptide (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) with an enterokinase-cleavage site; designed for antibody-mediated identification and purification of recombinant proteins.

体外研究

Fusion protein technology has become an important tool for solving numerous problems linked to recombinant protein production. The properties of the additional tag facilitate identification and provide a one-step purification procedure of the fusion protein by passing cell extracts or supernatants through columns of an appropriate matrix. FLAG peptide allows elution under non-denaturing conditions. Several antibodies against FLAG peptide have been developed. One antibody, M1, binds the peptide in the presence of bivalent metal cations, preferably Ca²⁺. Elution is effected by chelating agents. Another strategy is competitive elution with excess of free FLAGe peptide. Antibodies M2 and M5 are applied in this procedure. The Flag-tag is first described as a calcium-dependent epitope of a monoclonal antibody. It is a highly acidic octapeptide which can be N-terminally fused to the protein of interest. As a very hydrophilic peptide the Flag–tag has a high surface probability. Flag-fusion proteins can be captured by an immunoaffinity column in the presence of Ca²⁺ and eluted byEDTA at low concentrations, neutral pH and thus, nearly physiological conditions.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究

性状

Solid

溶解性数据

In Vitro:

H₂O : 100 mg/mL (98.72 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	0.9872 mL	4.9360 mL	9.8720 mL
5 mM	0.1974 mL	0.9872 mL	1.9744 mL
10 mM	0.0987 mL	0.4936 mL	0.9872 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶