Sulfosuccinimidyl oleate sodium Sulfo-N-succinimidyl oleate sodium|cas:1212012-37-7|西域试剂

Sulfosuccinimidyl oleate sodium Sulfo-N-succinimidyl oleate sodium,98.0%

产品编号：Bellancom-112847A| CAS NO：1212012-37-7| 分子式：C₂₂H₃₆NNaO₇S| 分子量：481.58

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货号	价格	库存与货期
Bellancom-112847A	￥1900.00	杭州北京（现货）
Bellancom-112847A	￥3200.00	杭州北京（现货）
Bellancom-112847A	￥13500.00	杭州北京（现货）

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Sulfosuccinimidyl oleate sodium Sulfo-N-succinimidyl oleate sodium

产品介绍

Sulfosuccinimidyl oleate sodium (Sulfo-N-succinimidyl oleate sodium) 是一种长链脂肪酸，抑制脂肪酸向细胞转运。Sulfosuccinimidyl oleate sodium 是一种有效且不可逆的线粒体呼吸链抑制剂。Sulfosuccinimidyl oleate sodium 结合小胶质细胞表面上的 CD36 受体。具有抗炎作用。

生物活性

Sulfosuccinimidyl oleate sodium (Sulfo-N-succinimidyl oleate sodium) is a long chain fatty acid that inhibits fatty acid transport into cells. Sulfosuccinimidyl oleate sodium is a potent and irreversible inhibitor of mitochondrial respiratory chain. Sulfosuccinimidyl oleate sodium binds the CD36 receptor on the surface of microglia. Anti-inflammatory effect.

体外研究

Sulfosuccinimidyl oleate (20 μM and 50 μM, 24 hours) alone does not alter the cellular viability. Exposure to 100 ng/ml LPS+5 ng/mL IFNγ modestly, yet significantly reduces the viability of the BV2 cells. Co-treatment with Sulfosuccinimidyl oleate prevents the LPS+IFNγ-induced reduction in the cell viability.
Sulfosuccinimidyl oleate (50 μM, 24 hours) co-treatment significantly reduces the LPS+IFNγ-induced expression of NOS2 and COX-2 in BV2 cells. Western blot analysis reveals a significant LPS/IFNγ-induced upregulation in the phosphorylated form of the p38, which is prevented by co-treatment with Sulfosuccinimidyl oleate (50 μM, 24 hours).

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay

Cell Line:	BV2 cells
Concentration:	20 μM and 50 μM
Incubation Time:	24 hours
Result:	Did not alter the viability of BV2 cells alone. Exposure of BV2 cells to 100 ng/mL LPS and 5 ng/mL IFNγ significantly reduced the viability of BV2 cells while simultaneous treatment with Sulfosuccinimidyl oleate prevented it.

Western Blot Analysis

Cell Line:	BV2 cells
Concentration:	50 μM
Incubation Time:	24 hours
Result:	Drastically increased the levels of NOS2, COX-2, and P-p38/T-p38.

体内研究
(In Vivo)

Sulfosuccinimidyl oleate (50 mg/kg; administered once by single oral gavage) significantly reduces the cortical ischemic infarct size compared to vehicle-treated controls in male BALB/cABom mice with pMCAo model. In addition, Sulfosuccinimidyl oleate at 50 mg/kg is suitable to see a beneficial effect after stroke.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	4-month-old male BALB/cABom mice with pMCAo model
Dosage:	50 mg/kg
Administration:	Administered once by single oral gavage
Result:	Reduced brain damage following ischemia. Attenuated infarct size.

体内研究

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	4-month-old male BALB/cABom mice with pMCAo model
Dosage:	50 mg/kg
Administration:	Administered once by single oral gavage
Result:	Reduced brain damage following ischemia. Attenuated infarct size.

体内研究

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	4-month-old male BALB/cABom mice with pMCAo model
Dosage:	50 mg/kg
Administration:	Administered once by single oral gavage
Result:	Reduced brain damage following ischemia. Attenuated infarct size.

性状

Solid

溶解性数据

In Vitro:

DMSO : 62.5 mg/mL (129.78 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	2.0765 mL	10.3825 mL	20.7650 mL
5 mM	0.4153 mL	2.0765 mL	4.1530 mL
10 mM	0.2076 mL	1.0382 mL	2.0765 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： 0.5% Methylcellulose/saline water
Solubility: 3.33 mg/mL (6.91 mM); Suspended solution; Need ultrasonic
2.

请依序添加每种溶剂： 50% PEG300 50% saline
Solubility: 3.33 mg/mL (6.91 mM); Suspended solution; Need ultrasonic
3.

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: ≥ 2.08 mg/mL (4.32 mM); Clear solution

此方案可获得 ≥ 2.08 mg/mL (4.32 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。
将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液
4.

请依序添加每种溶剂： 10% DMSO 90% corn oil
Solubility: ≥ 2.08 mg/mL (4.32 mM); Clear solution

此方案可获得 ≥ 2.08 mg/mL (4.32 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。