Aphidicolin 阿非迪霉素,99.0%

产品编号:Bellancom-N6733| CAS NO:38966-21-1| 分子式:C20H34O4| 分子量:338.48

Aphidicolin 是 DNA 聚合酶 α 和 δ 的抑制剂,通过干扰 DNA 聚合酶的活性来阻止有丝分裂细胞分裂。Aphidicolin 是 Cephalosporium aphidicola 产生的抗生素, 它是细胞脱氧核糖核酸合成的有效抑制剂,并且强烈抑制单纯疱疹病毒的生长。Aphidicolin 通过人早幼粒细胞白血病细胞系中阿拉伯糖基核苷诱导细胞凋亡(apoptosis)。

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货号 包装 价格 库存与货期 购买量 操作
Bellancom-N6733
1500.00 杭州 北京(现货)

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Aphidicolin 阿非迪霉素

产品介绍 Aphidicolin 是 DNA 聚合酶 α 和 δ 抑制剂,通过干扰 DNA 聚合酶的活性来阻止细胞有丝分裂。Aphidicolin 是一种来源于 Cephalosporium aphidicola 的抗生素, 能够抑制细胞脱氧核糖核酸合成和单纯疱疹病毒生长。Aphidicolin 具有抗正痘病毒活性,能够增强阿拉伯糖基核苷对细胞凋亡 (apoptosis) 的诱导作用。
生物活性

Aphidicolin is an inhibitor of DNA polymerase α and δ, prevents mitotic cell division by interfering DNA polymerase activity. Aphidicolin is an antibiotic produced by mold Cephalosporium aphidicola, inhibits cellular deoxyribonucleic acid synthesis and the growth of herpes simplex virus. Aphidicolin exhibits anti-orthopoxvirus activity and potentiates apoptosis induced by arabinosyl nucleosides in a human promyelocytic leukemia cell line.

体外研究

Aphidicolin (0.5 μM, 5 μM; 0-5 d) selectively kills neuroblastoma cells, but shows moderate cytotoxicity on normal human embryonal cells and HeLa, H9, A549 and Caco-2 cell lines[4].
Aphidicolin (0.4 μg/mL; 3 d) arrests cell cycle at G2 phase[5].
Aphidicolin (100 nM-10 µM; 48 h) inhibits cell proliferation via the p53-GADD45β pathway and (1 µM; 24 h) induces apoptosis in AtT-20 cells[6].
Aphidicolin (10 µM; 0-6 h) decreases the phosphorylation of Akt, (100 nM-10 µM; 24 h) increases the mRNA levels of the stress response gene growth arrest and DNA damage-inducible 45β (GADD45β), a putative downstream target of p53[6].
Aphidicolin (10 µM; 0-6 h) inhibits Varicella-zoster virus (VZV) with EC50s of 0.5-0.6 μM, with low cytotoxicity[7].

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Cytotoxicity Assay[4]

Cell Line: NB cell lines: UKF-NB-1/2/3 and IMR-32
Concentration: 0.5 μM, 5μM
Incubation Time: 1, 2, 3, 4, 5 days
Result: Resulted in cellular enlargement and extension of cellular processes before cell lysis occurred.

Cell Cycle Analysis[5]

Cell Line: Normal human diploid cells
Concentration: 0.4 μg/mL
Incubation Time: 3 days or 7 days
Result: Resulted more than 80% of the cells moved through S phase and were accumulated at G2 phase.
Inbihited the growth of the cells completely without causing apparent cell death.

Western Blot Analysis[6]

Cell Line: AtT-20 cells pituitary corticotroph tumor cells
Concentration: 10 µM
Incubation Time: 0 min, 5 min, 30 min, 2 h, 6 h, 24 h
Result: Inhibited Akt phosphorylation in AtT-20 cells during 5 min-2 h, in a time-dependent manner.
Increased protein level of p27 during 30 min-6 h, and remarkably increased p53 level at 24 h.
体内研究
(In Vivo)

Aphidicolin, has been developed with higher solubility agent, namely Aphidicolin glycinate (AG; NSC 303812).
Aphidicolin glycinate (100 mg/kg; i.p.; once every 3 h; 9 d) shows anti-tumor activity against the implanted B16 melanoma and M5076 sarcoma in murine, producing maximum increased life spans of 75%[8].

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: M5076 sarcoma s.c. model or B16 melanoma i.p. model in murine[8]
Dosage: 60 mg/kg, 100 mg/kg, 300 mg/kg
Administration: Intraperitoneal injection; once every 3 h; 9 days
Result: Inhibited tumor growth significantly.
体内研究

Aphidicolin, has been developed with higher solubility agent, namely Aphidicolin glycinate (AG; NSC 303812).
Aphidicolin glycinate (100 mg/kg; i.p.; once every 3 h; 9 d) shows anti-tumor activity against the implanted B16 melanoma and M5076 sarcoma in murine, producing maximum increased life spans of 75%[8].

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: M5076 sarcoma s.c. model or B16 melanoma i.p. model in murine[8]
Dosage: 60 mg/kg, 100 mg/kg, 300 mg/kg
Administration: Intraperitoneal injection; once every 3 h; 9 days
Result: Inhibited tumor growth significantly.
体内研究

Aphidicolin, has been developed with higher solubility agent, namely Aphidicolin glycinate (AG; NSC 303812).
Aphidicolin glycinate (100 mg/kg; i.p.; once every 3 h; 9 d) shows anti-tumor activity against the implanted B16 melanoma and M5076 sarcoma in murine, producing maximum increased life spans of 75%[8].

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: M5076 sarcoma s.c. model or B16 melanoma i.p. model in murine[8]
Dosage: 60 mg/kg, 100 mg/kg, 300 mg/kg
Administration: Intraperitoneal injection; once every 3 h; 9 days
Result: Inhibited tumor growth significantly.
性状Solid
溶解性数据
In Vitro: 

DMSO : 50 mg/mL (147.72 mM; Need ultrasonic and warming)

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.9544 mL 14.7719 mL 29.5438 mL
5 mM 0.5909 mL 2.9544 mL 5.9088 mL
10 mM 0.2954 mL 1.4772 mL 2.9544 mL
*

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效
储备液的保存方式和期限:-80°C, 6 months; -20°C, 1 month。-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式
Powder -20°C 3 years
In solvent -80°C 6 months
-20°C 1 month
参考文献

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危险品运输编码 NA 1993 / PGIII
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1. 物质的识别
产品名: Aphidicolin
CAS号: 38966-21-1
制造商/供应商: 西域试剂
网站:www.hzbp.cn   邮件:13911702513@139.com
2. 合成/成分数据
产品名: Aphidicolin
分子式: C20H34O4
分子量: 338.48
3. 急救措施
吸入后: 如果吸入,移至空气新鲜处,如果呼吸困难,给输氧,如呼吸停止,给予人工呼吸。
皮肤接触后: 用大量的水冲洗,移除污染的衣服和鞋子。
眼睛接触后: 检查并取下隐形眼镜,并用大量的水冲洗;呼叫医生。
吞食后: 如果吞食,用大量纯净水漱口;呼叫医生。
4. 消防措施
适当的灭火剂: 雾状水,二氧化碳,干粉或泡沫。
防护设备: 穿戴自给式呼吸器和防护服,以防止与皮肤和眼睛接触。
5. 泄漏应急处理
安全防范措施: 封锁泄漏区域;穿戴自给式呼吸器,防护服和厚橡胶手套。
清洁/收集措施: 使用液体粘合原料(硅藻土,通用粘合剂)吸取精细粉末;
使用酒精擦洗表面和设备除去污渍;
根据第11条处理被污染的材料。
6. 处理和储存
安全处理说明: 避免吸入和接触皮肤,眼睛及衣物;材料可能略微具有刺激性。
储存: 粉末型式       -20°C   3年;4°C   2年
溶于溶剂       -80°C   6个月;-20°C   1个月
7. 接触控制和个人防护
呼吸设备: NIOSH / MSHA认可的呼吸器。
双手保护: 耐化学腐蚀的橡胶手套。
眼睛防护: 化学安全护目镜。
8. 稳定性和反应活性
稳定性: 按照说明存储是稳定的;避免强氧化剂。
热分解/其他要避免的情况: 避免光和热。
9. 毒性资料
急性毒性: 无可用资料。
主要刺激性影响: 无可用资料。
在皮肤上: 无可用资料。
对眼睛: 无可用资料;可能具有刺激性。
10. 生态资料
一般注意事项: 无可用资料。
11. 废弃处置
按照所在国家,省份,县市和地方的法规处置。
12. 运输信息
正确的运输名称:
非危险品运输: 这种物质被视为非危险品运输。
13. 法规信息
尚未有针对此产品作出的化学安全性评估。
14. 其他信息
这种化学品仅供受过训练的,有经验的研究人员在穿戴适当装备和授权允许的情况下进行操作处理。以上信息基于我们目前的知识被认为是正确的,但只适用于作为有经验人员的指导。请咨询您自己的安全顾问,并遵守当地和国家的安全法规。在任何其他没有被警告的情况下,并不意味着绝对没有危险存在。西域生物技术不承担任何使用这种化学品所造成的损害和责任。2023 西域生物技术版权所有。





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