N1-Methylpseudouridine 1-Methylpseudouridine; N1-methyl-pseudouridine|cas:13860-38-3|西域试剂

N1-Methylpseudouridine 1-Methylpseudouridine; N1-methyl-pseudouridine,99.98%

产品编号：Bellancom-112582| CAS NO：13860-38-3| 分子式：C₁₀H₁₄N₂O₆| 分子量：258.23

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货号	价格	库存与货期
Bellancom-112582	￥500.00	杭州北京（现货）
Bellancom-112582	￥1000.00	杭州北京（现货）
Bellancom-112582	￥1600.00	杭州北京（现货）
Bellancom-112582	￥2600.00	杭州北京（现货）

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N1-Methylpseudouridine 1-Methylpseudouridine; N1-methyl-pseudouridine

产品介绍

N1-methyl-pseudouridine (1-Methylpseudouridine) 是一种甲基假尿苷，翻译性能优于 5 mC 和 5 mC/N1-methyl-pseudouridine。通过增加核糖体密度，mRNA 中的 N1-methyl-pseudouridine 以 eIF2α 依赖性和独立机制增强翻译。

生物活性

N1-methyl-pseudouridine (1-Methylpseudouridine), a methylpseudouridine, outperforms 5 mC and 5 mC/N1-methyl-pseudouridine in translation. N1-methyl-pseudouridine in mRNA enhances translation through eIF2α-dependent and independent mechanisms by increasing ribosome density.

体外研究

Incorporation of N1-methyl-pseudouridine into mRNA modifies mRNAs produced higher amounts of luc than the standard Luc mRNA in HEK293T cells. Incorporation of N1-methyl-pseudouridine nucleoside modification in both Luc and GFP mRNA enhances the initiation step of translation, in part by suppressing eIF2α phosphorylation. In addition, polysome formation and growth on the NN1-methyl-pseudouridine-containing Luc mRNA is enhanced due to the reduction of elongation rate. In all the in vitro translation systems, incorporation of N1-methyl-pseudouridine in Luc and GFP mRNAs dramatically enhanced translation. The N1-methyl-pseudouridine-Luc mRNA is associated with heavier polysomes than Luc mRNA.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究

N1-methylpseudouridine-incorporated mRNA outperforms pseudouridine-incorporated mRNA by providing enhanced protein expression and reduced immunogenicity in mammalian cell lines and mice.
N1-methyl-pseudouridine (1-Methylpseudouridine) (20 μg; I.m. or i.d. routes for 21 days) and m5C/ N1-methyl-pseudouridine-modified mRNA respectively have a higher translational capacity than Ψ and m5C/Ψ-modified mRNA in vivo.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	7-week-old Balb/c mice
Dosage:	20 μg
Administration:	I.m. or i.d. routes for 21 days
Result:	had a higher translational capacity.

体内研究

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	7-week-old Balb/c mice
Dosage:	20 μg
Administration:	I.m. or i.d. routes for 21 days
Result:	had a higher translational capacity.

性状

Solid

溶解性数据

In Vitro:

DMSO : 125 mg/mL (484.06 mM; Need ultrasonic)

H₂O : 50 mg/mL (193.63 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	3.8725 mL	19.3626 mL	38.7252 mL
5 mM	0.7745 mL	3.8725 mL	7.7450 mL
10 mM	0.3873 mL	1.9363 mL	3.8725 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： PBS
Solubility: 50 mg/mL (193.63 mM); Clear solution; Need ultrasonic
2.

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: ≥ 2.08 mg/mL (8.05 mM); Clear solution

此方案可获得 ≥ 2.08 mg/mL (8.05 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。
将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液
3.

请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in saline)
Solubility: ≥ 2.08 mg/mL (8.05 mM); Clear solution

此方案可获得 ≥ 2.08 mg/mL (8.05 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
4.

请依序添加每种溶剂： 10% DMSO 90% corn oil
Solubility: ≥ 2.08 mg/mL (8.05 mM); Clear solution

此方案可获得 ≥ 2.08 mg/mL (8.05 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。