KRN2 bromide|cas:1390654-28-0|西域试剂

KRN2 bromide,98.30%

产品编号：Bellancom-112125A| CAS NO：1390654-28-0| 分子式：C₂₇H₂₃BrFNO₄| 分子量：524.38

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货号	价格	库存与货期
Bellancom-112125A	￥900.00	杭州北京（现货）
Bellancom-112125A	￥1500.00	杭州北京（现货）
Bellancom-112125A	￥3100.00	杭州北京（现货）
Bellancom-112125A	￥5500.00	杭州北京（现货）
Bellancom-112125A	￥9900.00	杭州北京（现货）

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KRN2 bromide

产品介绍

KRN2 bromide 是选择性的 T 细胞激活核因子 (NFAT5) 的抑制剂，IC₅₀ 值为 100 nM。KRN2 bromide 有潜力用于 NFAT5-介导的慢性关节炎的潜能。

生物活性

KRN2 (bromide) is a selective inhibitor of nuclear factor of activated T cells (NFAT5), with an IC₅₀ of 0.1 μM.

体外研究

KRN2 selectively suppresses the expression of pro-inflammatory genes, including Nos2 and Il6, without hampering high-salt-induced NFAT5 and its target gene expressions.
KRN2 dose-dependently inhibits the NF-κB p65 binding to Nfat5 promoter 1 and directly blocks the interaction between NF-κB p65 and its DNA binding sequence in the upstream site (base pairs -3000 to +1) of Nfat5 exon 1.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究

KRN2 (3 mg/kg, i.p., daily for 2 weeks) effectively suppresses AIA in which innate immune cells play a predominant role.
KRN2 (3 mg/kg, i.p., daily) effectively suppresses CIA as well as AIA in mice, decreasing the production of pro-inflammatory cytokines and autoantibodies as well as macrophage infiltration.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	8-week-old C57BL/6 mice injected intradermally with 2 mg of complete Freund's adjuvant.
Dosage:	3 mg/kg.
Administration:	Peritoneally (i.p.) daily for 2 weeks.
Result:	Effective in suppressing AIA.

Animal Model:	8-week-old DBA/1Jmice immunized with bovine type II collagen.
Dosage:	3 mg/kg.
Administration:	Peritoneally (i.p.) daily.
Result:	Effective in suppressing CIA as well as AIA in mice, decreasing the production of pro-inflammatory cytokines and autoantibodies as well as macrophage infiltration.

体内研究

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	8-week-old C57BL/6 mice injected intradermally with 2 mg of complete Freund's adjuvant.
Dosage:	3 mg/kg.
Administration:	Peritoneally (i.p.) daily for 2 weeks.
Result:	Effective in suppressing AIA.

Animal Model:	8-week-old DBA/1Jmice immunized with bovine type II collagen.
Dosage:	3 mg/kg.
Administration:	Peritoneally (i.p.) daily.
Result:	Effective in suppressing CIA as well as AIA in mice, decreasing the production of pro-inflammatory cytokines and autoantibodies as well as macrophage infiltration.

性状

Solid

溶解性数据

In Vitro:

DMSO : 18.33 mg/mL (34.96 mM; Need ultrasonic)

H₂O : 1.1 mg/mL (2.10 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	1.9070 mL	9.5351 mL	19.0701 mL
5 mM	0.3814 mL	1.9070 mL	3.8140 mL
10 mM	0.1907 mL	0.9535 mL	1.9070 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: ≥ 1.83 mg/mL (3.49 mM); Clear solution

此方案可获得 ≥ 1.83 mg/mL (3.49 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 18.3 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。
将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液
2.

请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in saline)
Solubility: ≥ 1.83 mg/mL (3.49 mM); Clear solution

此方案可获得 ≥ 1.83 mg/mL (3.49 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 18.3 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
3.

请依序添加每种溶剂： 10% DMSO 90% corn oil
Solubility: ≥ 1.83 mg/mL (3.49 mM); Clear solution

此方案可获得 ≥ 1.83 mg/mL (3.49 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。
以 1 mL 工作液为例，取 100 μL 18.3 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。