HCH6-1|cas:1435265-06-7|西域试剂

HCH6-1,99.14%

产品编号：Bellancom-101283| CAS NO：1435265-06-7| 分子式：C₂₈H₂₇N₃O₄| 分子量：469.53

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货号	价格	库存与货期
Bellancom-101283	￥1400.00	杭州北京（现货）
Bellancom-101283	￥2100.00	杭州北京（现货）
Bellancom-101283	￥6100.00	杭州北京（现货）
Bellancom-101283	￥11000.00	杭州北京（现货）

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HCH6-1

产品介绍

HCH6-1 是一种有效的甲酰肽受体 1 (FPR1) 的竞争性二肽拮抗剂。HCH6-1 抑制 fMLF (FPR1激动剂) 特异性激活的人中性粒细胞的趋化性、超氧阴离子生成和弹性酶释放。动物实验中，HCH6-1 对急性肺损伤 (ALI) 具有保护作用，可用于 FPR1 参与的炎症性肺部疾病的研究。

生物活性

HCH6-1 is a potent and competitive dipeptide antagonist of Formyl peptide receptor 1 (FPR1). HCH6-1 inhibits chemotaxis, superoxide anion generation, and elastase release in human neutrophils specifically activated by fMLF (an FPR1 agonist). HCH6-1 has protective effects against acute lung injury (ALI) in vivo and can be used for the research of FPR1-involved inflammatory lung diseases.

体外研究

In a cell-impermeable cytochrome c reduction assay, HCH6-1 significantly inhibits superoxide anion generation in fMLF (FPR1 agonist)-activated neutrophils with an IC₅₀ of 0.32 μM. HCH6-1 has fewer inhibitory effects in WKYMVm (dual FPR1/FPR2 agonist)- and MMK1 (FPR2 agonist)-activated neutrophils, with IC₅₀s of 4.98±0.27 μM and 17.68±2.77 μM, respectively.
HCH6-1 does not induce LDH release even at 30 μM, so it does not have cytotoxic effects in human neutrophils. HCH6-1 does not alter the level of xanthine/xanthine oxidase superoxide anion and DPPH radical in cell-free systems.
HCH6-1 significantly inhibits elastase release in fMLF-activated neutrophils, with an IC₅₀ of 0.57 μM. However, in neutrophils triggered by WKYMVm or MMK1, HCH6-1 inhibits elastase release at higher concentrations, with IC₅₀s of 5.22±0.69 μM and 10.00±0.65 μM, respectively.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究

HCH6-1 (intraperitoneal injection; 50 mg/kg; 1 h before LPS spray or 30 min after LPS spray) alone does not induce airspace inflammation. HCH6-1 pretreatment reduces inflammatory cell infiltration and distortion of pulmonary architecture in the presence of LPS. HCH6-1 posttreatment shows inhibitory effects on neutrophil accumulation and lung damage in LPS-induced ALI mice.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	C57BL/6 mice (20-25 g, 7-8 weeks old)
Dosage:	50 mg/kg
Administration:	Intraperitoneal injection; 50 mg/kg; 1 h before LPS spray or 30 min after LPS spray
Result:	Ameliorated ALI in LPS-induced mice. HCH6-1-mediated decreasing of neutrophil recruitment serves as a protective mechanism in ALI mice.

体内研究

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	C57BL/6 mice (20-25 g, 7-8 weeks old)
Dosage:	50 mg/kg
Administration:	Intraperitoneal injection; 50 mg/kg; 1 h before LPS spray or 30 min after LPS spray
Result:	Ameliorated ALI in LPS-induced mice. HCH6-1-mediated decreasing of neutrophil recruitment serves as a protective mechanism in ALI mice.

性状

Solid

溶解性数据

In Vitro:

DMSO : 250 mg/mL (532.45 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	2.1298 mL	10.6489 mL	21.2979 mL
5 mM	0.4260 mL	2.1298 mL	4.2596 mL
10 mM	0.2130 mL	1.0649 mL	2.1298 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: ≥ 2.08 mg/mL (4.43 mM); Clear solution

此方案可获得 ≥ 2.08 mg/mL (4.43 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。
将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液
2.

请依序添加每种溶剂： 10% DMSO 90% corn oil
Solubility: ≥ 2.08 mg/mL (4.43 mM); Clear solution

此方案可获得 ≥ 2.08 mg/mL (4.43 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。