Apostatin-1 Apt-1|cas:2559703-06-7|西域试剂

Apostatin-1 Apt-1,99.24%

产品编号：Bellancom-134050| CAS NO：2559703-06-7| 分子式：C₁₉H₂₇N₃OS| 分子量：345.50

本网站销售的所有产品仅用于工业应用或者科学研究等非医疗目的,不可用于人类或动物的临床诊断或者治疗,非药用,非食用，

货号	价格	库存与货期
Bellancom-134050	￥900.00	杭州北京（现货）
Bellancom-134050	￥1500.00	杭州北京（现货）
Bellancom-134050	￥3000.00	杭州北京（现货）
Bellancom-134050	￥4800.00	杭州北京（现货）
Bellancom-134050	￥7800.00	杭州北京（现货）

增值税发票√顺丰快递√订货电话：18601927057

Apostatin-1 Apt-1

产品介绍

Apostatin-1 (Apt-1) 是一种有效的 TRADD 抑制剂。Apostatin-1 可与 TRADD-N 结合 (K_D=2.17 μM)，破坏其与 TRADD-C 和 TRAF2 的结合。Apostatin-1 调节 RIPK1 和 beclin 1 的泛素化。Apostatin-1 通过激活突变 tau、α-synuclein 或 huntingtin 积累的细胞中的自噬 (autophagy)，阻断凋亡 (apoptosis)，并恢复细胞内稳态。

生物活性

Apostatin-1 (Apt-1) is a potent TRADD inhibitor. Apostatin-1 can bind with TRADD-N (K_D=2.17 μM), disrupting its binding to both TRADD-C and TRAF2. Apostatin-1 modulates the ubiquitination of RIPK1 and beclin 1. Apostatin-1 blocks apoptosis and restores cellular homeostasis by activating autophagy in cells with accumulated mutant tau, α-synuclein, or huntingtin.

体外研究

Apostatin-1 inhibits Velcade (Bortezomib, HY-134050)-induced apoptosis and RIPK1-dependent apoptosis (RDA) and necroptosis, with an IC₅₀ of 0.97 μM.
Apostatin-1 (10 μM, 6 h) effectively induces autophagy and the degradation of long-lived proteins.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Western Blot Analysis

Cell Line:	SH-SY5Y, HeLa, HT-29, and Jurkat cells
Concentration:	10 μM
Incubation Time:	6 h
Result:	Effectively induced autophagy by LC3 II induction and p62 reduction.

体内研究
(In Vivo)

Apostatin-1 (20 mg/kg, IP, once) inhibits inflammatory responses, and increases survival of systemic inflammation mouse model.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Wild-type mice (n = 10, male, 8 weeks of age, systemic inflammation mouse model)
Dosage:	20 mg/kg
Administration:	IP, once
Result:	Reduced expression of the TNF-induced inflammatory target gene products, NOS and COXII27, and of inflammatory cytokines in cells stimulated with pathogen-associated molecular patterns, including interferon γ (IFNγ), lipopolysaccharide (LPS), Pam3CSK4 (a synthetic bacterial lipopeptide), and muramyl dipeptide (MDP). Showed increased survival following intravenous delivery of TNF, a mouse model of systemic inflammation.

体内研究

Apostatin-1 (20 mg/kg, IP, once) inhibits inflammatory responses, and increases survival of systemic inflammation mouse model.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Wild-type mice (n = 10, male, 8 weeks of age, systemic inflammation mouse model)
Dosage:	20 mg/kg
Administration:	IP, once
Result:	Reduced expression of the TNF-induced inflammatory target gene products, NOS and COXII27, and of inflammatory cytokines in cells stimulated with pathogen-associated molecular patterns, including interferon γ (IFNγ), lipopolysaccharide (LPS), Pam3CSK4 (a synthetic bacterial lipopeptide), and muramyl dipeptide (MDP). Showed increased survival following intravenous delivery of TNF, a mouse model of systemic inflammation.

体内研究

Apostatin-1 (20 mg/kg, IP, once) inhibits inflammatory responses, and increases survival of systemic inflammation mouse model.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Wild-type mice (n = 10, male, 8 weeks of age, systemic inflammation mouse model)
Dosage:	20 mg/kg
Administration:	IP, once
Result:	Reduced expression of the TNF-induced inflammatory target gene products, NOS and COXII27, and of inflammatory cytokines in cells stimulated with pathogen-associated molecular patterns, including interferon γ (IFNγ), lipopolysaccharide (LPS), Pam3CSK4 (a synthetic bacterial lipopeptide), and muramyl dipeptide (MDP). Showed increased survival following intravenous delivery of TNF, a mouse model of systemic inflammation.

性状

Solid

溶解性数据

In Vitro:

DMSO : 83.33 mg/mL (241.19 mM; ultrasonic and warming and adjust pH to 4 with HCl and heat to 80°C)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	2.8944 mL	14.4718 mL	28.9436 mL
5 mM	0.5789 mL	2.8944 mL	5.7887 mL
10 mM	0.2894 mL	1.4472 mL	2.8944 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式