Lefamulin acetate BC-3781 acetate|cas:1350636-82-6|西域试剂

Lefamulin acetate BC-3781 acetate,98.76%

产品编号：Bellancom-16908A| CAS NO：1350636-82-6| 分子式：C₃₀H₄₉NO₇S| 分子量：567.78

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货号	价格	库存与货期
Bellancom-16908A	￥2240.00	杭州北京（现货）
Bellancom-16908A	￥3360.00	杭州北京（现货）
Bellancom-16908A	￥6700.00	杭州北京（现货）

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Lefamulin acetate BC-3781 acetate

产品介绍

Lefamulin (BC-3781) acetate 是一种具有口服活性的抗生素 (antibiotic)。Lefamulin acetate 通过与 50S 细菌核糖体的肽基转移酶 peptidyl transferase 中心结合来抑制蛋白质合成。Lefamulin acetate 具有抗炎活性。Lefamulin acetate 可用于研究细菌感染，如细菌性肺炎。

生物活性

Lefamulin (BC-3781) acetate is an orally active antibiotic. Lefamulin acetate inhibits protein synthesis by binding to the peptidyl transferase center of the 50S bacterial ribosome. Lefamulin acetate has anti-inflammatory activity. Lefamulin acetate can be used in the research of bacterial infections, such as bacterial pneumonia.

体外研究

Lefamulin (0-1 mg/L) acetate shows inhibitory activity against C. trachomatis, N. gonorrhoeae, and M. genitalium.
Lefamulin acetate shows potent activity against all M. pneumoniae strains with MIC values of ≤0.008 μg/mL.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay

Cell Line:	C. trachomatis, N. gonorrhoeae, and M. genitalium
Concentration:	0-1 mg/L
Incubation Time:
Result:	Inhibited bacterial activity with MIC₅₀s of 0.02 mg/L, 0.063 mg/L and 0.12 mg/L respectively.

体内研究
(In Vivo)

Lefamulin (10-140 mg/kg, s.c.) acetate shows anti-inflammatory effect on LPS-induced lung neutrophilia mouse model^[4].
Lefamulin (1.25-160 mg/kg, s.c.) acetate shows antibacterial effect in S. pneumoniae or S. aureus challenged lung infection mice^[5].

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	LPS-induced lung neutrophilia mouse model^[4]
Dosage:	10-140 mg/kg
Administration:	Subcutaneous injection (s.c.)
Result:	Reduced BALF neutrophil cell counts. Reduced pro-inflammatory cytokine (TNF-α, IL-6, IL-1β, and GM-CSF), chemokine (CXCL-1, CXCL-2, and CCL-2) and MMP-9 levels in mouse lung tissue.

体内研究

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	LPS-induced lung neutrophilia mouse model^[4]
Dosage:	10-140 mg/kg
Administration:	Subcutaneous injection (s.c.)
Result:	Reduced BALF neutrophil cell counts. Reduced pro-inflammatory cytokine (TNF-α, IL-6, IL-1β, and GM-CSF), chemokine (CXCL-1, CXCL-2, and CCL-2) and MMP-9 levels in mouse lung tissue.

体内研究

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	LPS-induced lung neutrophilia mouse model^[4]
Dosage:	10-140 mg/kg
Administration:	Subcutaneous injection (s.c.)
Result:	Reduced BALF neutrophil cell counts. Reduced pro-inflammatory cytokine (TNF-α, IL-6, IL-1β, and GM-CSF), chemokine (CXCL-1, CXCL-2, and CCL-2) and MMP-9 levels in mouse lung tissue.

性状

Solid

溶解性数据

In Vitro:

DMSO : ≥ 100 mg/mL (176.12 mM)

H₂O : 1 mg/mL (1.76 mM; Need ultrasonic)

* "≥" means soluble, but saturation unknown.

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	1.7612 mL	8.8062 mL	17.6125 mL
5 mM	0.3522 mL	1.7612 mL	3.5225 mL
10 mM	0.1761 mL	0.8806 mL	1.7612 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： PBS
Solubility: 100 mg/mL (176.12 mM); Clear solution; Need ultrasonic
2.

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: ≥ 2.5 mg/mL (4.40 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (4.40 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。
将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液
3.

请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in saline)
Solubility: 2.5 mg/mL (4.40 mM); Clear solution; Need ultrasonic

此方案可获得 2.5 mg/mL (4.40 mM) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
4.

请依序添加每种溶剂： 10% DMSO 90% corn oil
Solubility: ≥ 2.5 mg/mL (4.40 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (4.40 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。