Silybin 水飞蓟宾|cas:802918-57-6|西域试剂

Silybin 水飞蓟宾,99.91%

产品编号：Bellancom-N0779A| CAS NO：802918-57-6| 分子式：C₂₅H₂₂O₁₀| 分子量：482.44

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货号	包装	价格	库存与货期	购买量	操作
Bellancom-N0779A		￥350.00	杭州北京（现货）
Bellancom-N0779A		￥665.00	杭州北京（现货）

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Silybin 水飞蓟宾

产品介绍

Silybin 是一种从草蓟 (Silybum marianum) 种子中分离出来的黄素寡糖。Silybin 诱导凋亡 (apoptosis)，并具有保肝，抗氧化，抗炎，抗癌活性。

生物活性

Silybin is a flavonolignan isolated from milk thistle (Silybum marianum) seeds. Silybin induces apoptosis and exhibits hepatoprotective, antioxidant, anti-inflammatory, anti-cancer activity.

体外研究

Silybin (0-200 mM; for 72 hours) has growth inhibition in a time- and dose-dependent manner.
Silybin (68 μM; for 72 hours) induces apoptosis and increases the cells in G1-phase of ~22%.
Silybin (68 μM; for 72 hours) induces AKT activity inhibition.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay

Cell Line:	HepG2 cell growth
Concentration:	0-200 mM
Incubation Time:	For 72 hours
Result:	Had growth inhibition in a time- and dose-dependent manner with an IC₅₀ of 68 μM.

Apoptosis Analysis

Cell Line:	HepG2 cell growth
Concentration:	68 μM
Incubation Time:	For 72 hours
Result:	Induced apoptosis in a higher number of cells (60%) when compared to untreated cells.

Cell Cycle Analysis

Cell Line:	HepG2 cell growth
Concentration:	68 μM
Incubation Time:	For 72 hours
Result:	Increased the cells in G1-phase of ~22% and decreased of 47% the cells in S-phase.

Western Blot Analysis

Cell Line:	HepG2 cell growth
Concentration:	68 μM
Incubation Time:	For 72 hours
Result:	Induced AKT activity inhibition.

体内研究
(In Vivo)

Silybin (50, 100 mg/kg/day; given intragastrically for the last 4 weeks) significantly lowers both serum and hepatic lipid accumulation.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male C57BL/6J mice (6-8 weeks old) with nonalcoholic fatty liver disease (NAFLD)
Dosage:	50, 100 mg/kg
Administration:	Given intragastrically; daily; for the last 4 weeks
Result:	Significantly lowered both serum and hepatic lipid accumulation.

体内研究

Silybin (50, 100 mg/kg/day; given intragastrically for the last 4 weeks) significantly lowers both serum and hepatic lipid accumulation.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male C57BL/6J mice (6-8 weeks old) with nonalcoholic fatty liver disease (NAFLD)
Dosage:	50, 100 mg/kg
Administration:	Given intragastrically; daily; for the last 4 weeks
Result:	Significantly lowered both serum and hepatic lipid accumulation.

体内研究

Silybin (50, 100 mg/kg/day; given intragastrically for the last 4 weeks) significantly lowers both serum and hepatic lipid accumulation.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male C57BL/6J mice (6-8 weeks old) with nonalcoholic fatty liver disease (NAFLD)
Dosage:	50, 100 mg/kg
Administration:	Given intragastrically; daily; for the last 4 weeks
Result:	Significantly lowered both serum and hepatic lipid accumulation.

性状

Solid

溶解性数据

In Vitro:

DMSO : 25 mg/mL (51.82 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	2.0728 mL	10.3640 mL	20.7280 mL
5 mM	0.4146 mL	2.0728 mL	4.1456 mL
10 mM	0.2073 mL	1.0364 mL	2.0728 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： 10% DMSO 90% corn oil
Solubility: ≥ 2.5 mg/mL (5.18 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (5.18 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。