L-161982|cas:147776-06-5|西域试剂

L-161982,99.04%

产品编号：Bellancom-108559| CAS NO：147776-06-5| 分子式：C₃₂H₂₉F₃N₄O₄S₂| 分子量：654.72

本网站销售的所有产品仅用于工业应用或者科学研究等非医疗目的,不可用于人类或动物的临床诊断或者治疗,非药用,非食用，

货号	价格	库存与货期
Bellancom-108559	￥900.00	杭州北京（现货）
Bellancom-108559	￥2500.00	杭州北京（现货）
Bellancom-108559	￥3900.00	杭州北京（现货）
Bellancom-108559	￥6900.00	杭州北京（现货）

增值税发票√顺丰快递√订货电话：18601927057

L-161982

产品介绍

L-161982 是一种选择性的 EP4 受体拮抗剂。L-161982 完全阻断 PGE2 诱导的 HCA-7 细胞 ERK 磷酸化和细胞增殖。L-161982 减轻胶原诱导的小鼠关节炎。

生物活性

L-161982 is a selective EP4 receptor antagonist. L-161982 completely blocks PGE2-induced ERK phosphorylation and cell proliferation of HCA-7 cells. L-161982 alleviates collagen-induced arthritis in mice.

体外研究

L-161982 (10 μM; 2 hours) blocks PGE2-stimulated cell proliferation of HCA-7 cells.
L-161982 (10 μM; 1 hour) blocks PGE2-stimulated ERK phosphorylation in HCA-7 cells.
L-161982 induces apoptosis, cell cycle arrest, and inhibits prostaglandin E2-induced proliferation in oral squamous carcinoma Tca8113 cells.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Proliferation Assay

Cell Line:	HCA-7 cells
Concentration:	10 μM
Incubation Time:	2 hours
Result:	Blocked PGE2-induced cell proliferation.

体内研究
(In Vivo)

L-161982 (5 mg/kg; i.p.; once per day for 2 weeks) reduces arthritis lesions and lesion progression in CIA mice.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Female DBA/1 mice of 6 to 8 weeks old (collagen-induced arthritis (CIA) mice model)
Dosage:	5 mg/kg
Administration:	i.p.; once per day for 2 weeks
Result:	Showed less joint swelling and lower arthritis score after 35 days post immunization.

体内研究

L-161982 (5 mg/kg; i.p.; once per day for 2 weeks) reduces arthritis lesions and lesion progression in CIA mice.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Female DBA/1 mice of 6 to 8 weeks old (collagen-induced arthritis (CIA) mice model)
Dosage:	5 mg/kg
Administration:	i.p.; once per day for 2 weeks
Result:	Showed less joint swelling and lower arthritis score after 35 days post immunization.

体内研究

L-161982 (5 mg/kg; i.p.; once per day for 2 weeks) reduces arthritis lesions and lesion progression in CIA mice.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Female DBA/1 mice of 6 to 8 weeks old (collagen-induced arthritis (CIA) mice model)
Dosage:	5 mg/kg
Administration:	i.p.; once per day for 2 weeks
Result:	Showed less joint swelling and lower arthritis score after 35 days post immunization.

性状

Solid

溶解性数据

In Vitro:

DMSO : 100 mg/mL (152.74 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	1.5274 mL	7.6369 mL	15.2737 mL
5 mM	0.3055 mL	1.5274 mL	3.0547 mL
10 mM	0.1527 mL	0.7637 mL	1.5274 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： 10% DMSO 90% corn oil
Solubility: ≥ 2.5 mg/mL (3.82 mM); Clear solution

此方案可获得 ≥ 2.5 mg/mL (3.82 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。
以 1 mL 工作液为例，取 100 μL 25.0 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。