Handelin|cas:62687-22-3|西域试剂

Handelin,99.44%

产品编号：Bellancom-N2083| CAS NO：62687-22-3| 分子式：C₃₂H₄₀O₈| 分子量：552.66

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货号	包装	价格	库存与货期	购买量	操作
Bellancom-N2083		￥2690.00	杭州北京（现货）
Bellancom-N2083		￥4570.00	杭州北京（现货）

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Handelin

产品介绍

Handelin 是来自Chrysanthemum boreale 的愈创木酚内酯二聚体，它通过下调 NF-κB 信号传导和促炎性细胞因子的产生而具有强大的抗炎活性。

生物活性

Handelin is a guaianolide dimer from Chrysanthemum boreale that has potent anti-inflammatory activity by down-regulating NF-κB signaling and pro-inflammatory cytokine production.

体外研究

Handelin (Compound 1; 10-40 μM; RAW 264.7 cells) treatment suppresses the LPS-induced (1 μg/mL) overexpression of iNOS and COX-2 protein levels in a concentration-dependent manner. Handelin also suppresseS the induction of pro-inflammatory cytokines TNF-α and IL-1β in LPS-stimulated RAW 264.7 cells. Handelin also suppresses the activation of mitogen-activated protein kinases, including ERK and JNK signaling.
Handelin (Compound 1; 10-40 μM; RAW 264.7 cells) treatment significantly reduces the iNOS and COX-2 mRNA levels in LPS- stimulated RAW 264.7 cells. The transcriptional activity of NF-κB stimulated with LPS is also suppressed by Handelin. In addition, the LPS-stimulated upregulation of miRNA-155 expression is suppressed by Handelin.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

RT-PCR

Cell Line:	RAW 264.7 cells
Concentration:	10 μM, 20 μM, 40 μM
Incubation Time:	5 hours
Result:	iNOS and COX-2 mRNA levels were significantly reduced. The transcriptional activity of NF-κB stimulated with LPS was also suppressed and the LPS-stimulated upregulation of miRNA-155 expression was also suppressed.

Western Blot Analysis

Cell Line:	RAW 264.7 cells
Concentration:	10 μM, 20 μM, 40 μM
Incubation Time:
Result:	Suppressed the LPS-induced overexpression of iNOS and COX-2 protein levels in a concentration-dependent manner. Suppressed the induction of pro-inflammatory cytokines TNF-α and IL-1β, and also also suppressed the activation of mitogen-activated protein kinases, including ERK and JNK signaling in LPS-stimulated RAW 264.7 cells.

体内研究
(In Vivo)

Handelin (Compound 1; 10-20 mg/kg; oral administration; for 30 mintues; male Sprague-Dawley rats) treatment inhibits acute inflammation in carrageenan-induced paw edema model. The serum level of IL-1β is also inhibited by Handelin in a carrageenan-induced paw edema model .

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male Sprague-Dawley (SD) rats (150-170 g, 5 weeks old) injected with carrageenan
Dosage:	10 mg/kg, 20 mg/kg
Administration:	Oral administration; for 30 mintues
Result:	Inhibited acute inflammation in carrageenan-induced paw. The serum level of IL-1β was also inhibited.

体内研究

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male Sprague-Dawley (SD) rats (150-170 g, 5 weeks old) injected with carrageenan
Dosage:	10 mg/kg, 20 mg/kg
Administration:	Oral administration; for 30 mintues
Result:	Inhibited acute inflammation in carrageenan-induced paw. The serum level of IL-1β was also inhibited.

体内研究

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male Sprague-Dawley (SD) rats (150-170 g, 5 weeks old) injected with carrageenan
Dosage:	10 mg/kg, 20 mg/kg
Administration:	Oral administration; for 30 mintues
Result:	Inhibited acute inflammation in carrageenan-induced paw. The serum level of IL-1β was also inhibited.

性状

Solid

溶解性数据

In Vitro:

DMSO : 16.67 mg/mL (30.16 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	1.8094 mL	9.0472 mL	18.0943 mL
5 mM	0.3619 mL	1.8094 mL	3.6189 mL
10 mM	0.1809 mL	0.9047 mL	1.8094 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (protect from light)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: 1.67 mg/mL (3.02 mM); Suspended solution; Need ultrasonic

此方案可获得 1.67 mg/mL (3.02 mM) 的均匀悬浊液，悬浊液可用于口服和腹腔注射。
以 1 mL 工作液为例，取 100 μL 16.7 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。
将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液
2.

请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in saline)
Solubility: 1.67 mg/mL (3.02 mM); Suspended solution; Need ultrasonic

此方案可获得 1.67 mg/mL (3.02 mM) 的均匀悬浊液，悬浊液可用于口服和腹腔注射。
以 1 mL 工作液为例，取 100 μL 16.7 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明
3.

请依序添加每种溶剂： 10% DMSO 90% corn oil
Solubility: ≥ 1.67 mg/mL (3.02 mM); Clear solution

此方案可获得 ≥ 1.67 mg/mL (3.02 mM，饱和度未知) 的澄清溶液，此方案不适用于实验周期在半个月以上的实验。
以 1 mL 工作液为例，取 100 μL 16.7 mg/mL 的澄清 DMSO 储备液加到 900 μL玉米油中，混合均匀。