ODN 2007|cas:455348-63-7|西域试剂

ODN 2007,93.51%

产品编号：Bellancom-150734| CAS NO：455348-63-7| 分子量：6852.50

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货号	包装	价格	库存与货期	购买量	操作
Bellancom-150734		￥5800.00	杭州北京（现货）
Bellancom-150734		￥12500.00	杭州北京（现货）

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ODN 2007

产品介绍

ODN 2007 为 B 类 CpG ODN (寡脱氧核苷酸), 是一种 Toll 样受体 (TLR) 配体。ODN 2007 可作为免疫调节剂、疫苗佐剂，增强哺乳动物、鱼类和人类的免疫反应。ODN 2007 序列：5'-TCGTCGTTGTCGTTTTGTCGTT-3'。

生物活性

ODN 2007, a class B CpG ODN (oligodeoxynucleotide), is a Toll-like receptor (TLR) ligand. ODN 2007 can be used as an immunomodulator, vaccine adjuvant, and enhance immune responses in mammals, fish, and humans. ODN 2007 sequence: 5'-TCGTCGTTGTCGTTTTGTCGTT-3'.

体外研究

ODN 2007 (1 or 5 μg/mL, 3-18 h) induces significantly chicken macrophage interferon IFN-γ and IFN-β expression.
ODN 2007 (10 μg/mL, 0.5-12 h) can increase levels of phosphorylation of ERK2 and AKT, stimulate the production of NO.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

RT-PCR

Cell Line:	MQ-NCSU cells (a chicken macrophage cell line)
Concentration:	1 μg/mL, 5 μg/mL
Incubation Time:	3 h, 12 h, 18 h
Result:	Significantly stimulated an increase in transcriptional expression of IFN-γ after 3 hours regardless of the dose. Increased the mRNA expression of IL-1β at 5 μg/mL high dose regardless of the time point.

Western Blot Analysis

Cell Line:	HD11, a replication-deficient avian leukemia virus MC29-transformed macrophage-like cell line
Concentration:	10 μg/mL
Incubation Time:	0.5 h, 1 h, 3 h, 6 h, 12 h
Result:	Resulted in a significant increase in ERK2 and AKT phosphorylation levels and increased IFN-γ, IL-6 and MIP-3α mRNA levels. Stimulated an increase in the level of NO.

体内研究
(In Vivo)

ODN 2007 (intraperitoneal injection, 1 μg, once) can enhance the immune response by regulating the expression of immune system-related genes in zebrafish infected withVibrio traumaticus FJ03-X2.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Zebrafish infected with Vibrio traumaticus FJ03-X2
Dosage:	1 μg
Administration:	Intraperitoneal injection; once
Result:	Showed a cumulative mortality rate of 15.0% in treated group while the control group reached 42.1%. Showed a significant decrease in the expression levels of all intestinal immune-related genes such as TNF、IFNg1-2、IL-1β、IL-10, especially IL-1β decreased by 99.88%.

体内研究

ODN 2007 (intraperitoneal injection, 1 μg, once) can enhance the immune response by regulating the expression of immune system-related genes in zebrafish infected withVibrio traumaticus FJ03-X2.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Zebrafish infected with Vibrio traumaticus FJ03-X2
Dosage:	1 μg
Administration:	Intraperitoneal injection; once
Result:	Showed a cumulative mortality rate of 15.0% in treated group while the control group reached 42.1%. Showed a significant decrease in the expression levels of all intestinal immune-related genes such as TNF、IFNg1-2、IL-1β、IL-10, especially IL-1β decreased by 99.88%.

体内研究

ODN 2007 (intraperitoneal injection, 1 μg, once) can enhance the immune response by regulating the expression of immune system-related genes in zebrafish infected withVibrio traumaticus FJ03-X2.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Zebrafish infected with Vibrio traumaticus FJ03-X2
Dosage:	1 μg
Administration:	Intraperitoneal injection; once
Result:	Showed a cumulative mortality rate of 15.0% in treated group while the control group reached 42.1%. Showed a significant decrease in the expression levels of all intestinal immune-related genes such as TNF、IFNg1-2、IL-1β、IL-10, especially IL-1β decreased by 99.88%.

性状

Solid

溶解性数据

In Vitro:

H₂O : 20 mg/mL (2.92 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	0.1459 mL	0.7297 mL	1.4593 mL
5 mM	---	---	---
10 mM	---	---	---

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式

-20°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

参考文献