Hexidium iodide, a fluorescent nucleic binding acid stain (excitation/emission ~ 518/600 nm), permeants to mammalian cells and selectively stains almost all gram-positive bacteria. Hexidium iodide can bind to the DNA of all bacteria after permeabilization by EDTA.

Hexidium iodide allows assessment of Gram status by differential absorption through bacterial cell walls, selectively staining gram-positive organisms in suspension.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

• . Shanmugam BK, et al. Biodegradation of tannery dye effluent using Fenton's reagent and bacterial consortium: A biocalorimetric investigation. J Environ Manage. 2019;242:106-113.  [Content Brief]

  . Holm C, et al. A flow-cytometric gram-staining technique for milk-associated bacteria. Appl Environ Microbiol. 2003;69(5):2857-2863.  [Content Brief]