Saikogenin D is isolated from Bupleurum chinense, has anti-inflammatory effects. Saikogenin D activates epoxygenases that converts arachidonic acid to epoxyeicosanoids and dihydroxyeicosatrienoic acids, and the metabolites secondarily inhibit prostaglandin E2 (PGE2) production. Saikogenin D results in an elevation of [Ca²⁺]i due to Ca²⁺ release from intracellular stores.

Saikogenin D (1-20 μM) inhibits PGE2 production induced by the Ca²⁺ ionophore A23187 in a concentration-dependent manner with an IC₅₀ value of 3 μM in C6 rat glioma cells.
Saikogenin D (10-100 μM) elevates [Ca²⁺]i in a concentration-dependent manner with a EC₅₀ value of 35 μM in the presence or absence of extracellular Ca2+ in C6 rat glioma cells.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

• . Toriniwa Y, et al. Participation of epoxygenase activation in saikogenin D-induced inhibition of prostaglandin E(2) synthesis.J Pharm Pharmacol. 2006 Jun;58(6):859-66.  [Content Brief]

  . Kodama Y, et al. Dual effect of saikogenin D: in vitro inhibition of prostaglandin E2 production and elevation of intracellular free Ca2+ concentration in C6 rat glioma cells.Planta Med. 2003 Aug;69(8):765-7.  [Content Brief]