Pseudothymidine is a C-nucleoside analog of thymidine.

Pseudothymidine is a C-nucleoside analog of thymidine. The calculated ΔΔG°₅₀/mod is -0.5 kcal/mol, with a ΔT_m/mod of 0.82°C. For the duplexes containing nine dA-T/ψT pairs, the ΔT_m/mod is -0.9°C and a ΔΔG°₅₀/mod is +1.1 kcal/mol. The modification of the duplex containing 12 consecutive dA-T/ψT base pairs produces a ΔT_m/mod of -0.9°C and a ΔΔG°₅₀/mod of +1.2 kcal/mol.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

• . S Lutz, et al. An in vitro screening technique for DNA polymerases that can incorporate modified nucleotides. Pseudo-thymidine as a substrate for thermostable polymerases. Nucleic Acids Res. 1999 Jul 1; 27(13): 2792-2798.  [Content Brief]

  . Havemann SA, et al. Incorporation of multiple sequential pseudothymidines by DNA polymerases and their impact on DNA duplex structure. Nucleosides Nucleotides Nucleic Acids. 2008 Mar;27(3):261-78.  [Content Brief]