KKL-35|cas:865285-29-6|西域试剂

KKL-35,99.47%

产品编号：Bellancom-101866| CAS NO：865285-29-6| 分子式：C₁₅H₉ClFN₃O₂| 分子量：317.70

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货号	包装	价格	库存与货期	购买量	操作
Bellancom-101866		￥900.00	杭州北京（现货）
Bellancom-101866		￥2250.00	杭州北京（现货）

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KKL-35

产品介绍

KKL-35是反式标记反应抑制剂，IC₅₀值为0.9 µM。

生物活性

KKL-35 is a trans-translation tagging reaction inhibitor with an IC₅₀ of 0.9 µM.

体外研究

KKL-35 exhibits broad-spectrum antibiotic activity. KKL-35 prevents growth of B. anthracis and M. smegmatis with minimum inhibitory concentration (MIC) values of less than 6 µM. KKL-35 inhibit trans-translation at some step before proteolysis of tagged proteins. KKL-35 inhibits tagging of DHFR-ns. A large amount of untagged DHFR is produced in reactions with the highest concentrations of KKL-35, indicating that KKL-35 does not inhibit translation. KKL-35 prevents growth of WT S. flexneri with a MIC of 6 µM, and addition of KKL-35 to a growing culture of S. flexneri stops growth. In an S. flexneri strain expressing ArfA and deleted for ssrA, addition of KKL-35 has little effect on viability or growth rate. KKL-35 inhibits the growth of E. coli ∆tolC, which is deficient in small molecule efflux, with an MIC of 0.3 µM.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究

Evidence suggest that the in vivo effects of KKL-35 are caused by inhibition of the release of nonstop translation complexes by trans-translation. KKL-35 inhibits trans-translation and prevents growth of S. flexneri strains that require trans-translation. The correlation between inhibition of trans-translation and growth is supported by genetic and pharmacological experiments showing that alternative mechanisms to release nonstop translation complexes relieve the growth suppression of KKL-35.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

体内研究

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

性状

Solid

溶解性数据

In Vitro:

DMSO : 6.25 mg/mL (19.67 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	3.1476 mL	15.7381 mL	31.4762 mL
5 mM	0.6295 mL	3.1476 mL	6.2952 mL
10 mM	0.3148 mL	1.5738 mL	3.1476 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

运输条件

Room temperature in continental US; may vary elsewhere.

储存方式