mTOR inhibitor-8|cas:2489196-70-3|西域试剂

mTOR inhibitor-8,98.04%

产品编号：Bellancom-131344| CAS NO：2489196-70-3| 分子式：C₂₄H₁₉ClN₄OS| 分子量：446.95

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货号	价格	库存与货期
Bellancom-131344	￥3000.00	杭州北京（现货）
Bellancom-131344	￥4800.00	杭州北京（现货）
Bellancom-131344	￥9500.00	杭州北京（现货）
Bellancom-131344	￥14500.00	杭州北京（现货）
Bellancom-131344	￥22500.00	杭州北京（现货）

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mTOR inhibitor-8

产品介绍

mTOR inhibitor-8 是一种 mTOR 抑制剂和自噬 (autophagy) 诱导剂。mTOR inhibitor-8 通过 FKBP12 抑制 mTOR 活性，并诱导 A549 人肺癌细胞自噬。

生物活性

mTOR inhibitor-8 is an mTOR inhibitor and autophagy inducer. mTOR inhibitor-8 inhibits the activity of mTOR via FKBP12 and induces autophagy of A549 human lung cancer cells.

体外研究

mTOR inhibitor-8 (Compound 5e; 0.1-10 μM; 24 and 48 hours) suppresses the growth of A549 cells in a dose-dependent manner.
mTOR inhibitor-8 induces autophagy in an mTOR-dependent manner. mTOR inhibitor-8 (10 μM; 3-24 hours) induces autophagy in a time-dependent manner. The levels of LC3B-II are enhanced.
mTOR inhibitor-8 (10 μM; 3-24 hours) reduces the phosphorylation of RPS6KB1 (ribosomal protein S6 kinase) and EIF4EBP1 (eukaryotic translation initiation factor 4E-binding protein 1), two essential substrates of mTOR

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Cell Viability Assay

Cell Line:	A549 cells
Concentration:	0.1, 1, 5, 10 μM
Incubation Time:	24 and 48 hours
Result:	Suppressed the growth of A549 cells with an IC₅₀ of 2.6±0.11 μM.

Western Blot Analysis

Cell Line:	A549 cells
Concentration:	10 μM
Incubation Time:	3, 6, 12 and 24 hours
Result:	The levels of phosphorylation of RPS6KB1 and EIF4EBP1 were significantly decreased after treatment.

体内研究
(In Vivo)

mTOR inhibitor-8 (25 and 50 μM; 6 days) effectively inhibits tumor growth in vivo without adverse effect on normal chick chorioallantoic membrane angiogenesis.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Fertile chicken eggs (7-9 days old)
Dosage:	25 and 50 μM
Administration:	6 days
Result:	Significant xenograft tumor remission was observed in eggs compared with the DMSO-treated eggs.

体内研究

mTOR inhibitor-8 (25 and 50 μM; 6 days) effectively inhibits tumor growth in vivo without adverse effect on normal chick chorioallantoic membrane angiogenesis.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Fertile chicken eggs (7-9 days old)
Dosage:	25 and 50 μM
Administration:	6 days
Result:	Significant xenograft tumor remission was observed in eggs compared with the DMSO-treated eggs.

体内研究

mTOR inhibitor-8 (25 and 50 μM; 6 days) effectively inhibits tumor growth in vivo without adverse effect on normal chick chorioallantoic membrane angiogenesis.

西域 has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Fertile chicken eggs (7-9 days old)
Dosage:	25 and 50 μM
Administration:	6 days
Result:	Significant xenograft tumor remission was observed in eggs compared with the DMSO-treated eggs.

性状

Solid

溶解性数据

In Vitro:

DMSO : 25 mg/mL (55.93 mM; Need ultrasonic)

配制储备液

浓度溶剂体积质量	1 mg	5 mg	10 mg

1 mM	2.2374 mL	11.1869 mL	22.3739 mL
5 mM	0.4475 mL	2.2374 mL	4.4748 mL
10 mM	0.2237 mL	1.1187 mL	2.2374 mL

请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month。-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。

In Vivo:

请根据您的实验动物和给药方式选择适当的溶解方案。以下溶解方案都请先按照 In Vitro 方式配制澄清的储备液，再依次添加助溶剂：

——为保证实验结果的可靠性，澄清的储备液可以根据储存条件，适当保存；体内实验的工作液，建议您现用现配，当天使用；以下溶剂前显示的百
分比是指该溶剂在您配制终溶液中的体积占比；如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶

1.

请依序添加每种溶剂： 10% DMSO 40% PEG300 5% Tween-80 45% saline
Solubility: 2.08 mg/mL (4.65 mM); Suspended solution; Need ultrasonic

此方案可获得 2.08 mg/mL (4.65 mM) 的均匀悬浊液，悬浊液可用于口服和腹腔注射。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 400 μL PEG300 中，混合均匀；向上述体系中加入50 μL Tween-80，混合均匀；然后继续加入 450 μL生理盐水定容至 1 mL。
将 0.9 g 氯化钠，完全溶解于 100 mL ddH₂O 中，得到澄清透明的生理盐水溶液
2.

请依序添加每种溶剂： 10% DMSO 90% (20% SBE-β-CD in saline)
Solubility: ≥ 2.08 mg/mL (4.65 mM); Clear solution

此方案可获得 ≥ 2.08 mg/mL (4.65 mM，饱和度未知) 的澄清溶液。
以 1 mL 工作液为例，取 100 μL 20.8 mg/mL 的澄清 DMSO 储备液加到 900 μL 20% 的 SBE-β-CD 生理盐水水溶液中，混合均匀。
将 2 g 磺丁基醚 β-环糊精加入 5 mL 生理盐水中，再用生理盐水定容至 10 mL，完全溶解，澄清透明